protocol similar to penicillin testing 4 weeks after the anaphylactoid reaction.ResultsTesting revealed a positive response to the arginine.ConclusionsThe use of intravenous arginine as a test of GH reserve remains safe and effective, but it is prudent to have the equipment and medication available ...
Figure 1. Experimental protocol. Period 1 indicates the study conducted with no maternal arginine supplementation, and period 2 is the study segment after the mother has received a primed infusion of arginine sufficient to raise the maternal plasma arginine concentration approximately 3-fold. Blood ...
The total level of acetyl-CoA and histone H3 acetylation were determined by ELISA assay kits according to Abcam manufacturer’s protocol (ab87546 and ab8115102, respectively). The individual complex activities were analyzed by ELISA assay kits according to Abcam manufacturer’s protocol (complex I:...
Elena Cattaneo for providing the pCAG-Htt1955-15Q construct and advice on the protocol for HTT immunoprecipitation. This work has been performed in the framework of Bando Progetti Strategici di Ateneo – University of Trento (M.P. and M.B.). This work was supported by grants from Telethon...
After washing with TBST, membranes were incubated with ECL-reagent (BioRad, cat. no. 1,705,061) according to the manufacturer’s protocol. Membranes were developed using standard X-ray films (AGFA, cat. no. EWPJH) or the chemiluminescence imaging system (Alliance Q9 Advanced, Uvitec, ...
Furthermore, after the starvation stimulation, the increase of GFP-LC3B-R68A puncta was much lower than that of GFP-LC3B, indicating that the LC3B-R68A mutant protein has lower autophagic activity in mammalian cells. Figure 5 Mutation of R68A impairs the autophagosomal localization and autophagic...
The per-protocol analysis excluded patients who died in the operating room, because these patients were deemed to have nonsurvivable injuries by a blinded panel of trauma surgeons and could not reach the end points of interest. Total complications were compared using a 2-tailed χ2 test. The ...
We refined the binding mode between KLF4 and PRMT5 by a five-step MD simulation protocol that incorporated all of the above-listed experimental data (See Modelling KLF4-PRMT5- binding section in SI); (i) first KLF4 was steered into the large cavity formed by the PRMT5 dimer so that the...
lymphoblastic leukemia (ALL) (n = 8). ThePvalue was determined using an unpaired two-sidedt-test (nrepresents the number of different cancer cell lines.c, Top: PRMTs mRNA levels in normal hematopoietic subsets from healthy donors (n = 7) or leukemia subsets from patients with ...
(2021). The protocol for this study was approved by the Local Ethics Committee (University of Warmia and Mazury, Olsztyn, Poland), and the animals were cared for under guidelines comparable to those laid down by the EU Directive 2010/63/EU (OJEU, 2010). Birds, management and diets In ...