Directed evolution of arginine deiminase for increased activity at physiological pHThe present invention relates to modified arginine deiminases exhibiting an increased activity at a higher pH, compared to a non-modified arginine deiminase, as well as uses of these enzymes for the treatment of cancer...
L(+)-Arginine's side chain is amphipathic, because at physiological pH it contains a positively charged guanidinium group, which is highly polar, at the end of a hydrophobic aliphatic hydrocarbon chain. Because globular proteins have hydrophobic interiors and hydrophilic surfaces, L(+)-Arginine is...
At physiological pH, these molecules are highly positively-charged, and are, therefore, unlikely to passively diffuse across the lipid bilayer. However, Hariton-Gazal et al. [87] clearly demonstrated that H1, H2A, H2B, H3 and H4 directly cross the plasma membrane of Hela and Colo-205 cells...
The arginine residue consists of a guanidino group on its side chain, which is protonated and positively charged at physiological pH3,5. The guanidino group forms multiple hydrogen bonds that bind with other interacting proteins or cofactors2,5. Although the methylated arginine residue retains its p...
Biochemistry A 'facultatively' essential amino acid that contains a guanido group with a pKa > 12, which carries a positive charge at physiological pH; it becomes an essential amino acid when the body is under stress or injured Sources Turkey, chicken and other meats. See Unproven methods for...
Apr 25 2023 What is the Function and Source of L(+)-Arginine? L(+)-Arginine is an amino acid that plays a crucial role in various physiological functions. It is encoded by specific codons and has a unique appear..12. Recommended SuppliersGlobal...
Each amino acid contain the same basic structure of a protonated amino group, an alpha carbon, and a de-protonated carboxyl group (at physiological pH). Each amino acid differs from one another in the structure of the R-group. Each amino acid will have a different...
4). However, because the pKa values of all guanidinium derivatives are so high, it is obvious that at physiological pH all are fully charged. Therefore, the small differences in basicity of the arginyl residue arising from methylation cannot have any physiological significance. A physiological role...
physiological amounts of arginine impair osteoclastogenesis. Hypoxia may induce iNOS expression in osteoclasts and promote osteoclastogenesis through NO-mediated effects [143,144]. In accordance, iNOS deficient mice were reported to have bone abnormalities associated with reduced osteoclast-mediated bone ...
hominis ADI, both at pH 6.4 (103 U/mg vs. 42 U/mg) and at physiological pH 7.4 (16.5 U/mg vs. 3.9 U/mg) in 37℃ [111, 112]. These findings hold promise for L. lactis ADI as an alternative therapeutic lead. Arginine deprivation may be beneficial for cancers with the presence ...