Polymerase chain reaction (PCR) was performed using a fluorometric quantitation Kit with a total of 20 µL. The volume of the 2×All-in-One™ qPCR Mix is 10 µL; the volume of the All-in-One™ miRNA qPCR Primer (2µM) is 2 µL; The universal Adaptor PCR Primer (2µ...
Spectrophotometric and fluorometric enzymatic assays were used to quantify arginine kinase substrates and products (Lowry and Passonneau, 1972). Equilibration was judged to have occurred when the [ATP][Arg]/[ADP][P–Arg] mass action ratio agreed to within 15%, when approached from both reaction...