Balancing the upper body is pivotal for upright and efficient gait. While models have identified potentially useful characteristics of biarticular thigh muscles for postural control of the upper body, experimental evidence for their specific role is lacking. Based on theoretical findings, we hypothesised...
Images were collected with a fluorescent inverted microscope (Olympus IX71) equipped with a CCD camera (Hamamatsu ORCA-R2). Cell length and area were evaluated using ImageJ software. Myocyte volume was then computed assuming an elliptical cross-section, in which the major axis corresponds to the ...
Total RNA was extracted from MSCs or SD-MSCs using the RNeasy Mini Kit (Qiagen; Valencia CA), and 500 ng was converted into cDNA with the RT2 First Strand Kit (SuperArray Bioscience Corp.; Frederick, MD). Real-time PCR was performed using the RT2 Angiogenesis PCR array with the RT2 SY...
Now imagine Eleanor lives her life again, but this time we induce in her the genetic or developmental atypicality that results in her developing DP. This causes severe real-world problems for those with DP, but our task is imperfect at capturing this, with the average DP level of impairment...
.eInduction of proinflammatory mediators by thrombin. Cytokines were measured by real-time PCR in astrocytes treated with thrombin (10 U/ml, 6 h) (n = 5 WT mice, 4 KO mice).f,gActivation of CRAC channels induces nuclear translocation of NFAT-GFP. WT (Orai1fl/fl) (f) or Ora...
Please note that infants viewed photographic images of real eyes (see Methods) and that the eye images shown here were computer generated with the FaceGen software (https://facegen.com) for illustrative purposes. Full size image Procedure Infants sat on their parents’ laps in a quiet, dimly...
during cell plating for 4-hrs. For analysis of invadopodia degradation and actin core size samples were imaged using a 63 × (NA 1.4) lens on a Leica DM-IRE2 upright microscope with a Leica TCS SP2 system (Leica, Heidelberg, Germany) and areas of degradation per cell or actin cores...
Germany) prior observation with Leica SP5 upright confocal laser-scanning microscope (Wetzlar, Germany). In some experiments, cells were not mounted but were directly observed with Zeiss LSM 780 inverted confocal laser-scanning microscope (Carl Zeiss AG, Jena, Germany). The images acquired under the...
Proteomic studies were performed on the Proteomics Platform PISSARO (plateau-proteomique.crihan.fr), IRIB, Faculty of Sciences, University of Rouen Normandy, 76821 Mont-Saint-Aignan, France, and in the Department of Medical Sciences of UCLM, Ciudad Real, Spain. Confocal microscopy images were ...
ICM founder cells are generated during or shortly after the fourth (the 8- to 16-cell transition) and fifth cleavage (the 16- to 32-cell transition) divisions4,6. The time between the completion of these divisions is approximately twelve hours32, during which outer-residing 16-cell stage bl...