<p>To determine the number of phosphodiester bonds in a plasmid DNA molecule with 1000 base pairs (bp), we can follow these steps:</p><p>1. <strong>Understanding the Structure of DNA</strong>: - DNA is composed of two strands that are complementary t
DNA Ligase:DNA ligase catalyzes the formation of phosphodiester bonds between DNA strands. There are different types of DNA ligases found in viruses, bacteria, and mammalian cells.DNA ligase plays a crucial role in Recombinant DNA technology, in vitro study of Molecular Biology and GeneticsAnswer an...
A) Hydrogen bonds B) Covalent bonds C) Phosphodiester bonds D) Polar covalent bonds The DNA double helix is held together by both strong and weak bonds. (a) True (b) False. Describe three types of DNA binding domains. Descri...
All these in vitro RBA1 substrates contain phosphodiester bonds, prompting us to test if DNA and RNA are RBA1 substrates. The purified RBA1 protein exhibited activity of degrading PCR product (Figure 1A, left), plasmid DNA (Figure 1A, right) or Arabidopsis genomic DNA (gDNA) (Figure 1B)....
1A). The MgA2+ ion is essential for recruitment, activation, and positioning of the nucleophilic water molecule that attacks the phosphodiester bond of the substrate, whereas MgB2+ stimulates product formation. Structural studies with Bacillus halodurans RNase H1 demonstrate that the MgA2+-binding ...
Rif inhibits RNAP function by blocking RNA translocation and extension after formation of the first or second phosphodiester bond41,44,45, resulting in inhibition of full-length transcript production along with an increase of abortive products (Fig.3e). By contrast, the Kangs inhibit the production...
In DNA: a. the AT base pair is formed through three hydrogen bonds and the GC base pair is formed through two hydrogen bonds. b. Watson and Crick identified the formation of phosphodiester bonds holding the two strands together. c. the replication of ...
DNA: Chemical Structure of Nucleic Acids & Phosphodiester Bonds from Chapter 9 / Lesson 1 121K Nucleic acids are large biomolecules used to store, transfer and convey genetic information in cells. Explore the structure of nucleotides and what polynucleotide and phosphodiester bonds do...
In the absence of a high resolution x-ray structure, we propose a tentative model for the cleavage complex involving a single-stranded DNA bubble containing the two scissile bonds and quinolone-binding sites (Fig. 8). We suggest that two quinolone molecules bind to the DNA phosphodiester backbon...
ionic bonds; scientists have not discovered where these are in the DNA helix c. hydro What is the bond between nucleotides in the DNA or RNA backbone called? (a) Peptide bond (b) Hydrogen bond (c) Phosphodiester linkage (d) Covalent bond. What type of bond is involved in the syn...