Many flaviviruses use viral apoptotic mimicry for cell entry (Supplementary Table1)5,6. Thus, we examined whether PS exposure is also critical for ZIKV infection. To this end, we enzymatically decarboxylated PS headgroups on virions (Fig.1a), a rapid process reaching a plateau within 5 min...
After this time, cells were washed four times with Krebs–Ringer MES (136 mM NaCl, 4.7 mM KCl, 1.25 mM CaCl2, 1.25 mM MgSO4, 10 mM MES, pH 6), three times with Krebs–Ringer phosphate (136 mM NaCl, 4.7 mM KCl, 1.25 mM CaCl2, 1.25 mM MgSO4, 10 mM NaH2PO4/Na2HPO4, pH 7.4) ...
Fig. 2: RNA-sequencing reveals that stimulus-evoked inflammatory pathways are reduced in Orai1 cKO astrocytes. Orai1fl/fl(WT) and Orai1fl/fl GFAP-Creastrocytes were stimulated with a low dose (0.2 µM) of TG + 50 nM PDBu (or DMSO) for 6 h and differentially expressed...
miR-214-5P downregulates RAD51 in BRCA wild-type/proficient TNBC cells We first determined whether expression levels of miR-214-5P and miR-142-3P negatively correlate with RAD51 expression in racially distinctive TNBC cell lines by performing RT-PCR analysis. As shown in Fig.3A, theRAD51levels...
22q11.2 deletion syndrome (22q11DS), a copy number variation (CNV) disorder, occurs in approximately 1:4000 live births due to a heterozygous microdeletion at position 11.2 (proximal) on the q arm of human chromosome 22 (hChr22) (McDonald-McGinn and Sull
Then they were perfused through the heart with perfusion buffer33 containing 0.1% sodium nitrite, followed by fixation with 4% paraformaldehyde in 0.1 mol/L phosphate buffer (PB) for 30 minutes. Brains were removed from the skulls and postfixed in the same fixative for 3 hours at room ...
PE belongs to the neutral phospholipid group, comprising the negatively charged phosphate group and positively charged ethanolamine. As aPLs mainly target negatively charged phospholipids, the antibodies developed against PE are less frequent [32]. Moreover, aPE positivity seems to be associated with AP...
Once in the plant, glyphosate inhibits, for example, the production of the enzyme EPSP (5-enolpyruvate-shikimo-3-phosphate) synthetase, which is responsible for the formation by plants of aromatic amino acids that are important for their growth and are included in the composition of many plant...
extracellular levels of MNV-1 were reduced by acute GW4869 treatment (Figure 3F) while replication was unaffected (Figure S3C), and MALDI-TOF/MS lipid analysis of the vesicles revealed the presence of bis(monoacylglycero)phosphate (BMP) (Figure 3G), a lipid enriched in MVBs and MVB-derived...
Using non- parametric (Spearman) correlation analysis (Table 2), it was observed that CD62L expression at day 8 negatively corre- lated (r = -0.4652; P = 0.00084) with TNF-α production in the co-culture system (n = 31). divided as compared with 2,126 in undivided cells; the MFI ...