Pick up any textbook with 'Microbiology' in the title and observe the scant to nonexistent treatment of animal cells in culture. Viruses do not suffer from such an exclusion. Chapters abound regaling the molecular niceties of the complex dances their components undergo while infecting bacteria or...
Poised enhancers (PEs) represent a genetically distinct set of distal regulatory elements that control the expression of major developmental genes. Before becoming activated in differentiating cells, PEs are already bookmarked in pluripotent cells with unique chromatin and topological features that could co...
J. ATAC‐seq: a method for assaying chromatin accessibility genome‐wide. Curr. Protoc. Mol. Biol. 109, 21.29.1–21.29.9 (2015). Article PubMed Google Scholar Künzli, M. et al. Long-lived T follicular helper cells retain plasticity and help sustain humoral immunity. Sci. Immunol. 5,...
1 Although the IUCN (International Union for Conservation of Nature) only recognizes a single species with nine subspecies,13 in recent years there have been proposals to classify giraffes into four separate species.4,5,6 High levels of genetic differentiation, even between directly neighboring ...
Fossilized Cells Found in China are Not Animal EmbryosKyle McDermott
“One advantage of using these stem cells is that they can proliferate all the time,” said Hangqi Luo, a postdoctoral associate in the lab. “So even one small cell can generate into thousands, tens of thousands of cells that we want.”...
Chromatin immunoprecipitation with an Sp1-specific antibody was performed on ST-treated T84 cells. Enhanced binding (∼2-fold) of Sp1 to the p21 proximal promoter was observed in ST-treated cells in comparison with untreated cells (Fig. 4C), and the increase in promoter occupancy was abrogated...
a process mediated by the upregulation of cyclin-dependent kinase inhibitors, such as p16INK4aand p21CIP11. Senescent cells also undergo multiple phenotypic changes, including altered morphology, chromatin remodelling, organelle reorganization, altered metabolism and the production of a bio-active secret...
Similar methods were used to track changes in these cells after antigen exposure. Our genome-wide analysis of the transcriptome and epigenome of iNKT cell subsets provides insights into the stability and plasticity of the chromatin landscapes that are initiated in the thymus and perhaps also ...
Interestingly, ToppCluster did not identify enrichment for any transcription factor binding sites in the most significant microarray genes, but utilizing the Animal Transcription Factor Database, we identified 37 transcription factors, 17 co-factors, and 7 chromatin remodeling factors differentially ...