The lower sensitivity of S2 was also observed in vitro (Fig. 3h). This result indicates that IDP ensemble sensitivity to changes in the cellular environment is encoded in sequence, but is difficult to predict since it may or may not correlate with the sensitivity measured in dilute buffers....
3h). This result indicates that IDP ensemble sensitivity to changes in the cellular environment is encoded in sequence, but is difficult to predict since it may or may not correlate with the sensitivity measured in dilute buffers. Biases in naturally occurring IDPs persist inside the cell Having...
It is prepared by mixing a weak acid and its conjugate base, or vice versa. When small amount of strong acid is added to the buffer, the conjugate base neutralizes the added acid; meanwhile, when small amount of strong base is added, the weak acid neutralizes the added base.Answer and...
can be cleaned with a variety of different acids. Acids will break the bonds between hematoxylin and the large proteins or even something on the glass such as protein that coats the slide with a blue tint. Strong acids would be used for a few seconds, while weak acids require extended ...
(amino acids 190–246) showed high specificity of binding to K48–K63-branched Ub4chains with no detectable binding to the unbranched Ub4controls (Fig.3dand Extended Data Fig.3a,b). Notably, the minimal UBD of RFC1 did not bind to K48–K63-branched Ub3. In contrast, the predicted ...
3e), equivalent to secondary dengue infections and influenza vaccination or infection23 and suggestive of enhanced affinity maturation24,25; thus, higher relative proportion (~80%) of dissimilar amino acids (Supplementary Fig. 4e). On average, the ZIKV-CR huMAbs accumulated more non-silent ...
Wash buffers were prepared by adding analytical-grade deionized water to the concentrated wash buffers. The kits were used according to the assay procedure. Combiwash Human ELISA plate washer was used for the washing processes. The enzyme-substrate reaction was terminated by adding a stop solution ...
buffers. The kits were used following the assay procedure. Washing processes were performed using the Combiwash Human ELISA plate washer. A stop solution was added to terminate the enzyme-substrate reaction and a rapid color change to yellow was detected. The optical density of each well at 450...
Cells were incubated in buffers of pH calibration buffer kit (Invitrogen), or additional pH buffers made by adjustments of pH with 1 mM NaOH (ChemSupply Australia), 10 μM of Valinomycin (Invitrogen) and 10 μM of Nigericin (Invitrogen) for 10 min before imaging. At least 200 ...
8). While there is some difference in the flanking sequence of the CxxC motif, the CxxC and CC motifs still align strongly across all eukaryotes, and the intervening length of the polypeptide between these regions only differs by a few amino acids in length (Supplementary Fig. 8). Thus, ...