The protein and RNA composition of supermeres differs from small extracellular vesicles and exomeres. Supermeres are highly enriched with cargo involved in multiple cancers (glycolytic enzymes, TGFBI, miR-1246, MET, GPC1 and AGO2), Alzheimer’s disease (APP) and cardiovascular disease (ACE2, ...
Pivotal in many ways for human health, the control of adult bone mass is governed by complex, incompletely understood crosstalk namely between mesenchymal stem cells, osteoblasts and osteoclasts. The SOX4, SOX11 and SOX12 (SOXC) transcription factors wer
transcriptional response to LPS was estimated as the gene expression difference between LPS and vehicle treatments. Because of the important role that HLA genes play in immune responses, and the complexity of the HLA region, we present all results for HLA genes separately at the end of the “Re...
2.5% (w/v) formaldehyde in 0.1 M cacodylate buffer (pH 7.0), respectively. The samples were post-fixed in buffered 1% (w/v) OsO4overnight. The samples were treated with 1% (w/v) uranyl acetate, dehydrated in a graded acetone series, and then embedded in...
[37,50]. Briefly, after discarding the first aliquot of blood to avoid contamination by keratinocytes, three aliquots of 3.0 mL of whole blood per sample were separately mixed with 4 mL of a proprietary red blood cell lysis and fixation buffer (ScreenCell®FC2 filtration buffer) and incubated...
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Root samples were collected from areas of new root growth (young root) and areas near the base of the stem (old root). Plant tissue was frozen and ground in liquid N2 in a pre-cooled mortar and pestle. Total soluble proteins were extracted using sodium phosphate buffer (100 mM NaPO4, ...
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Two controls (0.5 μg/ml and 5 μg/ml) were made in dilution buffer from Human CRP control material (code 85/506, NIBSC) and included in all runs. The LOD was 0.02 μg/ml. Concentrations below the LOD were assigned a random value between 0 and LOD extracted from a uniform ...
MES buffer Sigma Aldrich Cat# M3671 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 DMSO Sigma Aldrich Cat# 472301 Expi293 expression medium Thermo Cat# A1435101 ExpiFectamine 293 transfection kit Thermo Cat# A14524 Opti-MEM I medium Thermo Cat# 31985070 Filter cartridge UFP-10-C-4X2MA...