doi:10.1007/s002030100294Andreas NockerNila-Pia KrstulovicXavier PerretFranz NarberhausSpringer-VerlagArchives of MicrobiologyNocker, A., Krstulovic, N.P., Perret, X. and Narberhaus, F. 2001, ROSE elements occur in disparate rhizobia and are functionally interchangeable between species, Arch. ...
The presence of the expression cassette in the stable transformants was examined by PCR using corresponding primers and genomic DNA of stable transformants as a template. Fragments of predictable size (~ 3.3 kb) were obtained (data not shown). The level of FC b2 activity in cell-free ...
S-stimulated activity of lysates derived fromcrac−cells. Each of these supernatants restored the GTPγS-mediated activation of adenylyl cyclase to wild-type levels (data not shown). Taken together, these results show that the wild-type and GFP-tagged CRAC proteins are essentially interchangeable...
After elution, PCR was performed to amplify the 146 bp DNA fragment containing SBE1 at the rat α-SMA promoter using the primer pairs described above. Statistical Analysis Data are shown as means ± S.E.; n is the number of observations. We used z-tests to determine whether the ...
The presence of the expression cassette in the stable transformants was examined by PCR using corresponding primers and genomic DNA of stable transformants as a template. Fragments of predictable size (~ 3.3 kb) were obtained (data not shown). The level of FC b2 activity in cell-free ...