Finally, the pathway of F6P to Glc1P was enhanced by integrating another copy of glucose 6-phosphate isomerase (Pgi) and phosphoglucomutase (PgcA); a high a-arbutin titer (similar to 120 g/L) was obtained. The sucrose conversion rate was increased to 60.4% (mol/mol). In this study, ...
The separation was performed on a C 18 column with a 90:10 ( v / v ) mixture of 0.02M phosphate buffer and methanol as mobile phase. The effects of several conditions on HPLC resolution and CL emission were studied systematically. The linear ranges were 0.5–50 and 1–200μgmL 1 for ...