If sigma does not contact DNA near the -35 region, p(BAD) activity should be independent of the identity of the bases in the hexamer region that are not contacted by AraC. We have examined this issue in the p(BAD) promoter and in a second promoter where the AraC binding site overlaps...
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as described earlier.40 Determination of the MICs for toxic compounds The antibacterial activities of different agents were determined on Luria agar (1% tryptone, 0.5% yeast extract and 0.5% NaCl) plates containing various concentrations of the compounds (Sigma-Aldrich, St Louis, MO, USA) listed ...
sig22 (sav_3038) encodes an ECF sigma factor similar to S. coelicolor SigR, which controls the response to thiol-oxidative stress54. lexA (sav_2463) encodes a putative SOS regulatory protein. cspB1 (sav_3907), cspB2 (sav_3932), cspD1 (sav_826), cspD2 (sav_893), cspD3 (sav_4154...
Previous work established that the principal sigma factor (RpoV) of virulent Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex, resto... AJC Steyn,DM Collins,MK Hondalus,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 476...
The others are bacterial histone-like protein family, matp and sigma-54 factor family also transcription factor families that are binding candidate to MV6. H. seropedicae ACP92 genes are CpG Island which implies that the regulation of gene expression plays an important role.Mihret YirguMulugeta ...
The gels were subsequently stained with Sybr Green I according to the instructions of the supplier (Sigma) and photographed. Binding of DtxR to the ripA promoter was carried out in a 20-μl reaction mixture containing 100 mm Tris/HCl (pH 7.5), 5 mm MgCl2,40 mm KCl, 10% (v/v) ...
YASKAWA AC SERVO MOTOR SGMP-08A3TE11 。 SHIBAURA AC SERVO DRIVER CA10-S10G CA10S10G ORBOTECH ORBOTECH-DNV0-016087-REV-D ORBOTECHDNV0016087REVD FLOVEL AF/SIGMA I/O BOARD(20220A) CONTEC PI-48(98) PI48(98) ADVANTECH INDUSTRIAL ETHERNET SWITCH EKI-2525-AE EKI2525AE SANKYO AC SERVO MOTOR ...
AraC protein and the sigma subunit of RNA polymerase in Escherichia coli; Sensitivity of genetic transcription promoter activity to changes in bases not contacted by AraC; Indication that there exists only a slight degree of DNA binding cooperativity between AraC and the sigma subunit of RNA ...
Previous work has revealed that the system is controlled by two sigma factors, two negative regulators (cyclic AMP receptor protein [CRP] and H-NS), and an AraC-like regulator called GadX. Earlier evidence suggested that the GadX protein acts both as a positive and negative regulator of the...