thaliana ecotype Columbia-0 (Col-0) were obtained from the Arabidopsis Biological Resource Centre (ABRC), The Ohio State University, USA [42]. Arabidopsis seeds were sterilized using freshly prepared 5% calcium hypochlorite for 10 min and plated on half- strength MS medium supplemented with 1% ...
A. thaliana accession Columbia-0 (Col-0) was the background for all plants used in this study. WRKY33pro:EYFP-NLS (Col-0) and CBP60gpro:EYFP-NLS (Col-0) were generated as described in the subsection "Promoter-reporter imaging"66. 35Spro:GCaMP3 (Col-0)53, camta2 camta3 CAMTA3pro...
Arabidopsis thaliana, ecotype Columbia (Col-0) seeds were stratified at 4 °C in the dark for at least four days prior to sowing and growing on a growth medium consisting of a mixture of Jiffy plant starter pellets (Jiffy Products (NB) Ltd., New Brunswick, Canada) and vermiculite at ...
Arabidopsis thalianaColumbia-0 (Col-0) was used for all the experiments. TheArabidopsis thalianaT-DNA insertion lineprin2-2(GK-772D07-024643) was previously described24. Seedlings were grown on plates containing 1 × Murashige and Skoog (MS) salt mixture supplemented with vitamins (Duchefa M...
thaliana loci, the accession of the "Columbia" sequence from each of these loci was used for a local BLASTN search against A. lyrata sequences (using an e value threshold <1E-3): this procedure yielded 1118 ortholog sequences. After excluding the loci for which iden- tity was <0.85, ...
Herein, we evaluated the response of three natural ecotypes of Arabidopsis thaliana (L.) Heynh (Oasis, Columbia-0, and Chernobyl-07) to cadmium, using discovery gel-based proteomics. These accessions are differing by level of tolerance to heavy metal probably achieved by various exposure to ...
2.实验材料及方法 2.1 种子及幼苗培养 选取 Columbia 生态型野生型拟南芥(Arabidopsis thaliana)种子用 0.5%高锰酸钾 消毒 5 分钟,蒸馏水冲洗三遍后,置于 1%琼脂培养基上,在 HPG-400H 人工气候培养箱中 培养。培养箱中的光期温度为 23℃,暗期温度为 17℃;光照周期为 16/8h。种子萌发 5 天 后转移到培养土...
Using microarrays, we analysed whole-genome expression changes in Arabidopsis thaliana plants subjected to intraspecific competition. The leaf and root transcriptome was strongly altered by competition. Differentially expressed genes were enriched in genes involved in nutrient deficiency (mainly N, P, K),...
2 Arabidopsis thaliana male meiosis exposed to heat (30–32 °C) yields dyads, triads, and polyads. Quantitative analysis of the different types of male meiotic figures produced by Arabidopsis thaliana 2× Col-0 male sporogenesis exposed to varying periods of moderate heat stress (30–32 °C...
thaliana loci, the accession of the "Columbia" sequence from each of these loci was used for a local BLASTN search against A. lyrata sequences (using an e value threshold <1E-3): this procedure yielded 1118 ortholog sequences. After excluding the loci for which iden- tity was <0.85, ...