Here, seven X-ray crystallographic structures of the human nuclease apurinic/apyrimidinic (AP) endonuclease 1 (APE1) in complex with its substrate target flanked by a 5′ mismatch are reported. The structures reveal how APE1 influences the conformations of a variety of different mismatched base...
To evaluate apurinic/apyrimidinic sites (AP), one of the oxidative stress markers in normal and cancerous human endometrium and determine whether AP sites could be a molecular marker of endometrial cancer advancement.AP sites were investigated in DNAs of 33 endometrial cancer (EC) and 20 nonca...
After removal of the methylated base by methylpurine glycosylase, which creates the abasic or apurinic-apyrimidinic (AP) site, the phosphodiester bond is hydrolyzed immediately by AP endonuclease, initiating the repair of the AP site. ... L Liu,P Taverna,CM Whitacre,... - 《Clinical Cancer...
APEX1 Antibody is a Rabbit Polyclonal antibody against APEX1. Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pr
This study presents an innovative method for the highly sensitive detection of apurinic/apyrimidinic endonuclease 1 (APE1), a crucial biomarker and target for cancer diagnosis and treatment. The method is predicated on our discovery that the apurinic or apyrimidinic site (AP site) can inhibit ...
122 Kinetics of apurinic/apyrimidinic endonuclease 1 (APE1) on an authentic AP site or an AP site analog122 Kinetics of apurinic/apyrimidinic endonuclease 1 (APE1) on an authentic AP site or an AP site analogOur genomic DNA is endlessly exposed to a wide variety of exogenous and endogen...
Subsequently, the baseless site (apurinic/apyrimidinic site or AP site) is transformed into an SSB by the activity of an AP endonuclease, leaving a 3-hydroxyl and 5-phosphate gap that is filled by the DNA polymeraseβ (Pol β) (summarized in Ref. [116]). To date, 11 human damage-...
Harris2, Carmelo J. Rizzo2, Amanda K. McCullough1,3 & R. Stephen Lloyd1,4 Apurinic/apyrimidinic (AP) sites are constantly formed in cellular DNA due to instability of the glycosidic bond, particularly at purines and various oxidized, alkylated, or otherwise damaged ...
The effect of apurinic/apyrimidinic (AP) sites in DNA on RNA and protein synthesis was studied in vitro using T7 coliphage DNA. Initiation of RNA synthesis by Escherichia coli RNA polymerase was synchronized and heparin was used to prevent reinitiation. When the T7 DNA contained AP sites,...
AP site A site in DNA where a base is missing (AP site=apurinic site ordepending on the nature of the missing base). base analog Chemical mutagen that mimics a DNA base. Mutation in which one base is replaced by another. chain termination mutation ...