Effects of aPLs IgG on monocytes were studied by measuring the surface expression of tissue factor (TF) and excretion of TNF-alpha from peripheral blood mononuclear cell culture. Results and conclusion: Thrombotic complications among SLE patients were closely associated with aCL/beta(2)GPI or aPS/...
T-cell proliferation and cytokine assay Peripheral blood mononuclear cells were isolated from healthy peripheral hu- man blood by Ficoll gradient centrifugation. Monocytes were removed by plastic adherence for 2 h in RPMI-1640 Medium (Roswell Park Memorial Institute)+10% fetal calf serum (FCS) at ...
Enzyme-linked immunosorbent analysis (ELISA) Be- fore the rats were killed, 1.5 mL of blood was collected from each rat and centrifuged immediately to remove the blood cells. Concentrations of IFN-γ and IL-4 in the animal sera were estimated by commercially available ELISA kits (R&D), ...