for instance as described in U.S. Pat. No. 4,683,202 or Saiki et al.,Science239 (1988), 487-491. A more recent review of PCR methods may be found inPCR Protocols,1990, Academic Press, San Diego, Calif., USA.
Statistical analysis of normalised gene expression data before and after treatment was performed using two-tailed unpaired t tests (GraphPad Prism version 3.02 Software, San Diego, Calif., USA). Statistical significance was indicated by a P value ≦0.05. The aim of this study was to determine wh...
Statistics and data analysis In the binding tests, non- linear curve fitting by GraphPad PRISM 4.0 (San Diego, CA, USA) was used to generate affinity (Kd) and capacity (Bmax) values for [3H]-QNB and the competition parameters. The apparent dissociation constants (Ki) were calculated from ...
The primary antibody against cyclin B, Cdc-2, p53, p21, and Bcl-2, all belong to the mouse antihuman monoclonal antibody (Imgenex Co, San Diego, CA, USA). The secondary antibody (Jackson Immuno Research Lab Inc, West Grove, PA, USA) conjugated with horseradish peroxidase was added at ...
30、iety onthe Abuse of Children, San Diego, CA.Ruby, J., & Fulton, C. (1993, June). Beyond redlining: Editing softwaret works.ter sespresented at the annual meeting of the Society for Scholarly Publishing, Washington, DC.15 团体著者且是者名称用 AuthorAustralian Bureau of Sistics. (...
Saturation and displacement curves were ana- lyzed by non-linear regression analysis with Prism (GraphPad Software, San Diego, CA, USA). Statistical analysis Data from confocal quantity were analyzed using the unpaired t-test with statistical signifi- cance defined as P<0.05. Data from Western ...
Table 2 and Table 3 report- ing the trial pool of descriptors used in the MDCK cell per- meation MI-QSAR modeling use both classifications of the descriptors. The general intramolecular solute descriptors included as part of the trial descriptor pool are defined in Table 2. The term "general...
Erythrocytes were lysed by am- 1072 Http://www.chinaphar.com Zhang M et al monium chloride (BD Bioscience, San Diego, CA, USA). Cells were resuspended in supplemented RPMI-1640 and counted on a hemocytometer in trypan blue to ensure viability. After being stimulated with PMA, ionomycin and...
The cells were then stained with fluo- rescein-isothiocyanate-conjugated anti-TLR9 mAb or an unconjugated rabbit anti-TLR7 polyclonal antibody (Imgenex, San Diego, CA, USA). PE-conjugated goat antirabbit immunoglobulin G (Molecular Probes, Eugene, OR, USA) was used as a secondary antibody for...