Advances in whole genome amplification and next-generation sequencing methods have enabled genomic analyses of single cells, and these techniques are now beginning to be used to detect genomic lesions in individual cancer cells. Previous approaches have been unable to resolve genomic differences in compl...
Ch. 8 (The Royal Society of Chemistry, 2022). Eisenstein, M. Illumina faces short-read rivals. Nat. Biotechnol. 41, 3–5 (2023). CAS PubMed Google Scholar Arslan, S. et al. Sequencing by avidity enables high accuracy with low reagent consumption. Nat. Biotechnol. 42, 132–138 (...
Currently, Illumina (short-read), PacBio (long-read), and Oxford Nanopore (long-read) are the most popular sequencing technologies. Unlike PacBio or the popular short read sequencers before it, which, as examples of the second or so-called Next Generation Sequencing platforms, need to ...
Just as Sanger sequencing ushered in a paradigm shift that enabled the molecular basis of biological questions to be directly addressed, to an even greater degree, ultra-high-throughput DNA sequencing is poised to dramatically change the nature of biological research. New sequencing technologies have ...
Errors during the emPCR amplification step can lead to biases and incorporation mistakes, affecting the overall quality of the sequencing data. The advent of simpler and faster sequencing technologies like Illumina and Oxford Nanopore has led to a decline in the use of SOLiD sequencing. ...
While several sequencing platforms are available, Illumina has emerged as the primary tools in most studies due to low cost per base, high-throughput, and low error rates. To further save costs and increase throughput, single-cell libraries are often barcoded and pooled together for multiplexed ...
Over the past decade, long-read, single-molecule DNA sequencing technologies have emerged as powerful players in genomics. With the ability to generate reads tens to thousands of kilobases in length with an accuracy approaching that of short-read sequencing technologies, these platforms have proven ...
Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling. The revolution from bulk RNA sequencing to single-molecular, single-cell and spatial transcriptome approaches has enabled i
In the realm of non-coding RNA (ncRNA) research, computational biology plays a pivotal role. As an interdisciplinary field that leverages computational science techniques to probe and comprehend biological phenomena, computational biology
Illumina-sequencing 5. Data Analysis The final step is bioinformatics analysis of the sequencing data. The sequencing reaction produces reads stored in FASTQ files which are demultiplexed to separate samples by barcodes and remove the barcodes using tools like sabre. ...