This chapter focuses on the application of transgenic mice to address various problems of skeletal biology such as the effects of protein structure and gene regulation on bone structure or bone cell differentiation. Transgenic mice provide an excellent setting for studying complex regulatory systems that...
or temporal expression3,4. In studies using BAC Tg mice, BAC transgene integrity and copy number are not always described in detail. Here, we developed a simple method to investigate the genomic organisation and integration sites of transgenes using a combination of droplet digital PCR (ddPCR) ...
[21], using a transgenic mouse model which spontaneously develops asthma, confirmed some of the results. In this study, Ym1 and Ym2 were upregulated both at the protein and mRNA level, comparing control versus asthmatic mice. After dexamethasone treatment, Ym1 mRNA levels decreased whereas Y...
involves various unique phenotypic alterations in cells [32,33]. Despite the proportion of senescent cells in the vascular system of the elderly is generally low, several mechanisms of cellular senescence have been demonstrated that may damage vascular function and promote the progression...
Characterisation of the effects of nicotine in the five-choice serial reaction time task in rats: antagonist studies. Psychopharmacology (Berl.) 149, 293–305 (2000). Article CAS Google Scholar Lambourne, S.L. et al. Impairments in impulse control in mice transgenic for the human FTDP-17 ...
Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component of stem cell potency, ov
Friedman RA, Ryan AF (1992) Transgenic mice. Current applications to the study of the auditory and vestibular systems. Otolaryngol Clin North Am 25:1017–1026 CAS Frykholm C, Larsen H, Dahl N, Klar J, Rask-Andersen H, Friberg U (2006) Familial Ménière’s disease in five generations. Ot...
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. Furthermore, Jiang et al. applied light-reactive bacterial Cry2 protein to generate oligomers of tau-Cry2 chimeras in primary cortical neurons in mice with AD and investigated the interactions between tau oligomers, RNA-binding proteins, and N6-methyladenosine RNA. The study showed that light-...
Characterization of the transgenic mice that broadly express MetRS L274G To enable the in vivo labeling of mammalian proteome via broad expression of mMetRSL274G, we crossed floxed STOP-EGFP tagged MetRSL274G mice (i.e., fx mice) with CMV-Cre mice (Fig. 1a). Fx mice were generated ...