This article describes the experimental conditions that should be applied to avoid molecular degradation in size-exclusion chromatography of polymers of ultra-high molar mass (weight-average molar mass M w>5000 kg/mol). The applicability of the optimized experimental conditions is demonstrated using ...
Size exclusion chromatographymolecular weight distributionintrinsic viscosity distributionviscosity detectorlight scattering detectorbrominated polystyrenèA brominated polystyrene sample was used as a probe to explore the increased capabilities of size exclusion chromatography (SEC) with on-line light scattering and...
5.3.5Size-Exclusion Chromatography Gradients Application ofchromatographyat limiting conditions requires selection of a suitable barrier solvent and width, allowing penetration of one type ofmacromolecules, while the barrier should be sufficiently weak to hold back the other polymer type. This limits the ...
In any embodiment of the present application, in step 2), the purification is selected from a combination of one or more high-performance liquid chromatography, size exclusion chromatography, and gel electrophoresis. A third aspect of the present application provides the drug conjugate of the first ...
Size exclusion chromatography (SEC) coupled with a multi-angle light scattering (MALS) detector is demonstrated as the most efficient method of the determination of molar mass distribution. The number average molar masses determined by SEC-MALS are compared with those measured by vapor phase ...
size-based separation, polymer precipitation, and immunoaffinity chromatography (Fig.3A) [57,58]. However, the variability of exosomes in terms of their size, content, function, and origin complicates their separation. Most current techniques struggle to completely separate them from lipoproteins or ...
[0144] The molecular weight and molecular weight distribution can be used to characterize a polymer sample. Gel permeation chromatography (“GPC”), more correctly termed “size exclusion chromatography,” can be used for characterization of molecular weight and distribution of molecular weight in the...
Chromatography Process Analytical Technology (PAT) Quality Control/Quality Assurance (QA/QC) Quality by Design (QbD) Regulatory Standards, GLP and GMP Compliance Sample Preparation Size-Exclusion Chromatography (SEC) Solid-Phase Extraction (SPE) Supercritical Fluid Chromatography (SFC) Supercritical Fluid ...
5.4. Size exclusion chromatography (SEC) SEC is a technique utilized to separate molecules based on their size. The method involves the passage of an herbal solution through a stationary phase consisting of porous resin particles. As particles of varying sizes exhibit distinct retention times, SEC ...
connected to silica tubings and placed on a heating plate (32 °C). PBS buffer pH 7.4 was used as release medium and was pumped with a speed of 0.5 ml/h through the cells with a multi-syringe pump. The release fractions were collected and analysed by size exclusion chromatography....