We used the FACSAria III Cell Sorter (BD Biosciences, San Jose, CA) to sort single cells treated with CCR5-Uco-hetTALEN (or non-treated cells as control) into a 96-well PCR plate containing 10 µl of lysis buffer (50 mM Tris base pH 8.0, 10 mM EDTA, 100 mM NaCl, 0.1...
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Nucleic acid is the main material for storing, copying, and transmitting genetic information. Gene sequencing is of great significance in DNA damage research, gene therapy, mutation analysis, bacterial infection, drug development, and clinical diagnosis.