The non-protein part cofactor together with the protein part apoenzyme forms a holoenzyme. Apoenzymes are important for enzymatic activity since they are responsible for the specificity of enzymes to their substrates. Apoenzymes alone are not active enzymes; they must bind to an organic or ...
, although the enzymatic activity was reconstituted by the interaction of the apoenzyme binding site with the long spacered PQQ immobilized as a monolayer on the electrode surface, there was no direct electron transfer between the electrode surface and the PQQ redox site inside the holoenzyme. ...