公司主营elisa试剂盒,抗体,生物试剂及进口品牌sigma,abcam,invitrogen,CST,roche等经销 详细信息 人载脂蛋白E(ApoE)酶联免疫吸附测定试剂盒主要参数: 英文名称 Human ApoE (Apolipoprotein E) ELISA Kit 中文名称 人载脂蛋白E(ApoE)酶联免疫吸附测定试剂盒 货号 E-EL-H0470 种属 Human/人 规格 96T/Kit (8*12...
brain protein concentration, the latter determined in a neat brain homogenate using the bicinchoninic acid assay (Pierce Biotechnology Inc.; Rockford, IL). The concentration of Aβ oligomers was determined in the neat homogenate using a human aggregated β-amyloid ELISA kit (Invitrogen, Carlsbad, CA...
1.1 材料 小鼠 MMP��9 ELISA 试剂盒购于上海西唐生物科技有限公 司;Trizol 购于 Invitrogen 公司;逆转录试剂盒(RevertAidTM First Strand cDNA Synthesis Kit)购于 Fermentas 生物公司;SYBR�k Premix Ex TaqTM RT��PCR Kit 购自 TaKaRa 公司;油红 O 购自美国 Amresco 公司;天狼星红和 FITC 标记...
The levels of Aβ42 in cultured media 2 days after treatment were measured by human Aβ42 ELISA kit (Invitrogen) following the manufacturer’s instructions. Reduced levels of Aβ42 by iPSC-derived astrocytes were calculated by subtracting remaining Aβ42 from total Aβ42. Aβ42 uptake index...
After washing, we incubated the sections with the goat anti-rabbit IgG (H + L) AF647 antibody and goat anti-rat IgG (H + L) AF488 antibody (Invitrogen; 1:1,000 dilution) overnight at 4 °C. We acquired confocal images using a Zeiss LSM880 confocal microscope. During ...
the sections were incubated with a mixture of Alexa Fluor 488- and 594-coupled secondary antibodies (Invitrogen) for detection. DAPI (1 μg/mL) (Sigma) was used for staining nuclei. Images were acquired with an Olympus confocal FV1000 imaging system. ImageJ (NIH) was used for intensity ...
After washing three times with PBS, samples were incubated with fluorescently conjugated secondary antibodies (Alexa Fluor 488 and 594 conjugates, Invitrogen, 1:500) for 2 h at room temperature and washed three times with PBS before mounting with the glass coverslip. Fluorescent signals were ...
To visualize the vasculature, 0.1 ml BBB impermeable Texas Red-dextran 70 (MW 70kD; 1% in saline, Invitrogen) was injected intravenously immediately before imaging. A Mai Tai laser (SpectraPhysics) attached to a confocal scanning system (Fluoview 300, Olympus) and an upright microscope (IX51W...
To reduce dye associated cytotoxicity, astrocytes were incubated with 0.5 µM Fluo-4-AM (Invitrogen) and 0.02% Pluronic™ F–127 in DMEM for 15 min at 37 °C. Cells were then washed once with HEPES-buffered Tyrode’s solution (Alfa Aesar), and allowed to de-esterify at RT...
(Invitrogen, Cat#15596-026) and reverse transcribed to cDNA using FastQuant RT kit (TIANGEN, Cat#KR106). qRT-PCR was carried out on the Light Cycler 480 System (Roche) using the FastStart Universal SYBR Green Master (Roche, Cat#04913850001) and primers targeting specific genes, including ...