Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed.9) All samples should be disposed of in a manner that will inactivate viruses.10) Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should ...
Storage of components varies. See protocol for specific instructions. Background: Apolipoprotein A-I/ApoA1 Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse...
根据《currentprotocolsinimmunology》中操作流程,使用人apoa1重组蛋白与等体积的弗氏完全佐剂乳化,对8周龄的balb/c小鼠进行免疫,然后用弗氏不完全佐剂乳化,共免疫四次,每次免疫注射量为200μg蛋白。 2.3杂交瘤细胞的制备、筛选和单克隆化 分离免疫的balb/c小鼠的脾细胞,与小鼠骨髓瘤细胞sp2/0融合。通过elisa方法,对...
AAV2-human apoA1(WT), AAV2-human apoA1(4WF), and AAV2-eGFP were manufactured by the University of Pennsylvania Vector Core using the triple plasmid transfection protocol59. Human apoA1 ELISA assay was purchased from R&D Systems (Minneapolis, MN, USA). WST-8 assay kit was purchased from ...
(L3000075, Thermo Fisher Scientific) and cultured under standard culture conditions for 48 h according to the manufacturer’s protocol. The cells were then washed with PBS and lysed with cell lysis buffer (100 μL/well). The lysate was centrifuged at 10,000–15,000 rpm for 3–5 min, ...