Enzymeヽatalyzed proximity labeling (PL) with the engineered ascorbate peroxidase APEX2 is a novel approach to map organelle compartmentalization and protein networks in living cells. Current procedures developed for mammalian cells do not allow delivery of the co﹕ubstrate, biotin﹑henol, into ...
APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1. APEX2 also permits superior enrichment of endogenous mitochondrial and endoplasmic reticulum membrane proteins. This is a preview of ...
VAPBVesicle-associated membrane protein鈥揳ssociated protein B (VAPB) is a tail-anchored protein that is present at several contact sites of the endoplasmic reticulum (ER). We now show by immunoelectron microscopy that VAPB also localizes to the inner nuclear membrane (INM). Using a modified ...
APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1. APEX2 also permits superior enrichment of endogenous mitochondrial and endoplasmic reticulum membrane proteins. 展开 ...
研究胰岛素相互作用蛋白及优化APEX2互补片段检测蛋白相互作用的任务书任务名称:研究胰岛素相互作用蛋白及优化APEX2互补片段检测蛋白相互作用背景:胰岛素是一种重要的激素,参与调节血糖水平和脂肪代谢等生理过程。胰岛素与其相互作用的蛋白是了解胰岛素调控机制的关键。同时,蛋白相互作用的研究对于揭示细胞内分子网络、疾病机制亦...
Dynamic protein-protein interactions (PPIs) play crucial roles in cell physiological processes. The protein-fragment complementation (PFC) assay has been developed as a powerful approach for the detection of PPIs, but its potential for identifying protei
After 48 h, the supernatant was harvested, filtered through a low-protein binding HT Tuffryn® membrane with 0.45 μm pores (Pall cat. #4184), and stored at −80°C. Lentiviral titer was determined by transducing the cell line of interest plated at clonogenic density with serial dilution...
NCBI Protein IDNP_084219.1 Ensembl Transcript IDENSMUST00000026303.16 Ensembl Gene IDENSMUSG00000025269.17 Ensembl mRNA IDApex2-201 包装清单: 产品编号产品名称包装 QM45686SMouse Apex2 qPCR Primer Pair1nmol each —说明书1份 保存条件: -20℃保存。建议复溶后进行适当分装,避免反复冻融。
DirectedevolutionofsplitAPEX2peroxidaseYisuHan1,2,3,4,TessCarolineBranon1,2,3,4,5,JeffreyD.Martell1,6,DanielaBoassa7,DavidShechner8,MarkH.Ellisman7,..
Reminiscent of their evolutionary origin, mitochondria contain their own genome (mtDNA) compacted into the mitochondrial chromosome or nucleoid (mt-nucleoid). Many mitochondrial disorders are characterized by disruption of mt-nucleoids, either by direct