We introduce APEX-seq, a method for RNA sequencing based on spatial proximity to the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome, revealing extensive and exquisite patterns of localization for diverse RNA...
The transcriptome of A549 cells with and without APEX1 over expression was determined by Illumina sequencing, followed by analysis of AS. RT-qPCR validated expression of APEX1-related genes in A549 cells. We have successfully applied RNA-seq technology to demonstrate APEX1 regulation of AS. ...
Suitable Downstream AnalysisPCR/qPCR, Sequencing, Mass Spec Volume (Metric) ProcessingKF 96 plate 15 to 200 μL, 96 DW plate 15 to 1000 μL, 96 storage tube 30 to 200 μL Plates per Deck8 Format96-well Capacity96 Samples/Run DescriptionKIngFisher Apex with 96 Combi head Collection Efficien...
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Suitable Downstream AnalysisPCR/qPCR, Sequencing, Mass Spec Volume (Metric) Processing96 KF plate 50 to 150 μL, 96 DW plate 50 to 1000 μL Plates per Deck8 Format96-well Capacity96 Samples DescriptionKingFisher Apex with 96 DW head
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exposure. In the final stage of the training, two of the four groups displayed an above-chance level performance on a numerical sequencing task.
An MD5 checksum provides data integrity through hashing and sequencing to ensure that data is not altered or stolen as it is transmitted over a network. Syntax APEX_ITEM.MD5_CHECKSUM( p_value01 IN VARCHAR2 DEFAULT NULL, p_value02 IN VARCHAR2 DEFAULT NULL, ...
Coomassie and sequencing grade modified trypsin were purchased from Promega. Streptavidin magnetic beads were purchased from Pierce (Catalog No. 88817). Construction of incremental truncations and interaction protein pairs The initial N- and C-terminal fragments of APEX2 were amplified from PCDNA3-flag...
An MD5 checksum provides data integrity through hashing and sequencing to ensure that data is not altered or stolen as it is transmitted over a network. Syntax APEX_ITEM.MD5_CHECKSUM( p_value01 IN VARCHAR2 DEFAULT NULL, p_value02 IN VARCHAR2 DEFAULT NULL, ...