3、RT-PCR克隆拟南芥AP1基因(1)逆转录反应(根据TaKaRa公司的RT-PCR试剂盒说明进行操作)按下列组成在反应管中配制反应液10×RNA PCR缓冲液2μlMgCl2(25mmol/L) 4μldNTP混合物(各10mmol/L) 2μlAMV Reverse Transcriptase XL(5U/μl) 1μlRNase Inhibitor(40U/μl) 0.5μlOligo(dT)20Primer(2.5μmol/L...
双荧光素酶报告基因实验结果显示,miR-NC组、miR-195-5p组共转染WT-AFAP1-AS1后相对荧光素酶活性比较差异有统计学意义(P<0.05),共转染MUT-AFAP1-AS1后相对荧光素酶活性比较差异无统计学意义(P>0.05)。与inhibitor-NC+si-NC组比...
Pre-treatment with the activator protein-1 (AP-1) inhibitor curcumin attenuated TNF-α induced transcription of the TGF-β1 gene. TNF-α induced increased levels of c-Jun and C-Fos in the nucleus accompanied by phosphorylation of c-Jun. In electrophoretic mobility shift assays, AP-1 binding...
In addition, the notion that blockade of canonical KRAS signaling leads to adaptive reactivation of the signaling pathway resulted in a concept of low-dose vertical inhibition, which includes the combination of a pan-RAF inhibitor together with an ERK inhibitor. This combination impeded ERK ...
⽣物素标记EMSA探针－AP1是⽤于EMSA(也称gel shift)研究的并经⽣物素(Biotin)标记的AP1 consensus oligonucleotide。这个⽣物素标记的双链寡核苷酸含有公认的AP1结合位点，可以⽤作EMSA研究时的探针。AP1 consensus oligo的序列如下：5’-CGC TTG ATG ACT CAG CCG GAA-3’3’-GCG AAC TAC TGA GTC GGC ...
生物素标记EMSA探针－AP1是用于EMSA(也称gel shift)研究的并经生物素(Biotin)标记的AP1 consensus oligonucleotide。这个生物素标记的双链寡核苷酸含有公认的AP1结合位点，可以用作EMSA研究时的探针。AP1 consensus oligo的序列如下：5’-CGC TTG ATG ACT CAG CCG GAA-3’3’-GCG AAC TAC TGA GTC GGC CTT-5’...
联系人: 王羽 手机: 传真: 地址: 上海上海市 在线询盘 详细信息alpha Adaptin/AP1??衔接蛋白α抗原蛋白质及多肽合成?0.5mg/1mg本公司主营抗体、一抗、标记一抗、标记二抗、蛋白质及多肽合成,由于篇幅所限,更多抗体及信息请致电详询!alpha Adaptin/AP1??衔接蛋白α抗原更多相关产品:DB4243ICAD(Inhibitor of Cas...
1c). To confirm the association of happen of autophagy flux and DOX resistance, T-47D and MDA-MB-453 cells were treated with the lysosome acidification inhibitor Bafilomycin-A1 (Baf-A1) or autophagosome-lysosome fusion inhibitor chloroquine (CQ). The results showed that when treated with DOX ...
手机: 传真: 地址: 上海上海市 在线询盘 详细信息alpha Adaptin/AP1??衔接蛋白α抗原蛋白质及多肽合成?0.5mg/1mg本公司主营抗体、一抗、标记一抗、标记二抗、蛋白质及多肽合成,由于篇幅所限,更多抗体及信息请致电详询!alpha Adaptin/AP1??衔接蛋白α抗原更多相关产品:DB4243ICAD(Inhibitor of Caspase-Activated ...
Curcumin(c-Jun/AP-1inhibitor).On another aspect ofthe cis・ element,the experiment ofDNAaseI Footprint Wasusedtodeterminethe binding ofAP-1and enhancersites.Therelative activity ofluciferaseWasalsotestedafter dealing theMsC withCurcuminortransfectionofthe ...