MiR-137通过JNK/AP-1信号通路调控低强度剪切应力诱导的人主动脉内皮细胞内质网应激和细胞凋亡。 综上所述,该研究证明了miR-137在剪切应力刺激的HAECs中的潜在调节作用,可以通过靶向SDS22调控JNK/AP-1信号通路来促进细胞氧化应激反应和细胞凋亡。研究提供了证据支持miR-137在介导剪切应力急性反应中的关键作用,这可能为...
15 000 r/min 离心 10 min,取上清。在 10% SDS 聚丙烯酰胺凝胶上进行 电泳 ,电泳结束后转至硝 酸纤维素膜上 ,用含质量分数 5% 脱脂奶粉的 TBS/T 封闭 1 h,在封闭液 中加 入 1:2000 稀释的 一 抗,4 ℃过夜 ,用 TBS/T 冲洗 3 次 ,加 入辣 根过 ...
Various lysates were subjected to SDS PAGE followed by western blot with 18985-1-AP (iNOS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.View All Images (6)Featured Product KD/KO Validated iNOS Polyclonal antibody iNOS Polyclonal Antibody for WB, IF/ICC, FC (...
WB analysis using 17785-1-AP Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.View All Images (28)Synaptophysin Polyclonal antibodySynaptophysin Polyclonal Antibody for WB,...
应用Positive Control; Immunogen; SDS-PAGE; WB 更多产品信息 重组人WBP1蛋白丨Recombinant human WBP1 protein 重组小鼠SOST蛋白丨Recombinant Mouse SOST protein 重组小鼠SEMA4C蛋白丨Recombinant Mouse SEMA4C protein 重组人APOA2蛋白丨Recombinant human APOA2 protein ...
2. 去垢剂SDS以及螯合剂EDTA可以抑制RVC的活性,请勿和RVC同时使用。 储存条件:-20℃,有效期一年。 关注c-Fos/activator protein(AP)-1抑制剂(530141-72-1)(98.41%),的同时,为您推荐更多您可能感兴趣的产品: YT933 BET抑制剂(GSK525762) 10mM×0.2ml|5mg|25mg M00231 缺氧诱导因子-1α抑制剂(HIF-...
SDS loading buffer was added to the resin and the mixture was boiled for 5 min. The samples were then centrifuged briefly. Five microlitres of supernatant was analysed by SDS–PAGE. The protein bands were visualized by Coomassie blue staining. For AP-1 ΔμCTD pull-down, 30 μg GST...
Cells were lysed in modified RIPA buffer (50 mM Tris, 150 mM NaCl2, 1% Triton X-100, 0.75% SDS, 0.5% sodium deoxycholate), which was supplemented with protease and phosphatase inhibitor cocktail (Roche). Cellular extracts were sonicated using a Covaris B208 ultrasonicator to produce ...
Tested Applications Product Information Publications (1) Protocols Print datasheet MSDS Validation Data Gallery Filter: AllWBIHCIF/ICC WB analysis of NIH/3T3 using 16685-1-AP NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16685-1-AP (CRK Antibody) at dilution of ...
Cat no :19483-1-AP Synonyms NES Print datasheet MSDS Validation Data Gallery Filter: AllWBIHCIF/ICCFC (Intra) WB analysis using 19483-1-AP Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room te...