construct encoding a short-hairpin activator protein-1 binding polynucleotide (shAP-1 binding polynucleotide), said shAP-1 binding polynucleotide comprising an activator protein 1 (AP-1) binding site sequence and a reverse complement of said AP-1 binding site sequence separated by a spacer sequence....
Site-specific mutations in the AP-1 consensus sequence, which resulted in the complete loss of binding to cellular factors, eliminated the basal activity and reduced the inducible promoter activity by eightfold. 展开 关键词: Humans Cytomegalovirus Cells, Cultured DNA, Viral Immediate-Early Proteins ...
DNA-methylation is known to repress transcription either by inactivation of positive regulatory cis-elements containing CpG dinucleotides or via the sequence-nonspecific and methylation-specific binding of inhibiting methyl-CpG dinucleotides or via the sequence-nonspecific and methylation-specific binding of ...
A luciferase assay using deletion constructs of the cyclin D1 promoter indicated that E2-induced increase in transcriptional activity was observed in reporters containing AP-1-binding site sequence, and that in the absence of E2, cotransfection of c-Jun also showed increase of transcriptional ...
RNA immunoprecipitation (RIP) was used to analyze RNA molecules binding with RacGAP1. To detect active RhoA, immunoprecipitation of active RhoA was performed with the RhoA Activation Assay Kit. Details of the materials and methods were shown in Supplementary Materials 1. Primer sequences are listed...
Resminostat (Fig.7) is a type of histone deacetylase (HDAC) inhibitor that can competitively bind to AP-1/DNA-interacting target sequences and effectively block their downstream signaling pathways. HDAC inhibitors can affect the deacetylation state of c-Jun, thereby regulating c-Jun activity and...
Extension of this site to a 7-nt AP-1 sequence massively promoted latency establishment, suggesting that this promoter region represents a latency establishment element (LEE). Given that these minimal changes in a transcription factor binding site affect latency establishment to such large extent, ...
The F promoter, containing the sequence analyzed here, is one of the multiple promoters of the human ERalpha gene and is the only active promoter in bone tissue. Through electrophoretic mobility shift (EMSA), chromatin immunoprecipitation (ChIP), and re-ChIP assays, we investigated the binding ...
L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10, R25 (2009). Article PubMed PubMed Central CAS Google Scholar Bhattacharyya, S., Chandra, V., Vijayanand, P. & Ay, F. Identification of significant chromatin contacts from HiChIP data...
Sp1 and Smad3 as well as c-Jun were found bound to the WT AP-1 elements, but Smad3 and Sp1 binding to the mSp1 sequence was considerably reduced whereas c-Jun binding remained constant. Additional co-IP assays confirm the interaction of Sp1, c-Jun and Smad3 (data not shown) as ...