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SH-SY5Y cells were passaged every 2–3 days and differentiated into a neuron-like state using a 5-day differentiation protocol with all-trans-retinoic acid (MedChemExpress, #HY-14649) as described previously33. For assessment of ATG9A translocation, differentiated SH-SY5Y cells were plated in ...
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Transcript quantification was performed using RSEM (v1.3.0) (Li and Dewey, 2011) against all 26,000 genes in NCBI RefSeq database (version date; 2015-07-17). Surrogate variables are covariates constructed directly from high-dimensional mRNA-Seq data that could be used in subsequent analyses ...
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Crop breeding heavily relies on natural genetic variation. However, additional new variations are desired to meet the increasing human demand. Inflorescence architecture determines grain number per spike, a major determinant of bread wheat (Triticum aest