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In lieu of an abstract, here is a brief excerpt of the content: html_title /html_title Reviewed by Robert Laurie (bio) The History of Natural Hi... R Laurie - 《Library the Transactions of the Bibliographical Society》 被引量: 1发表: 2011年 The Astrophysical Journal: A New Journal for...
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RT-qPCR experiments were performed using the CFX Connect PCR Detection System (Bio-Rad, United States) and Q3 SYBR qPCR Master Mix (TOLOBIO). For all samples, three biological replicates and three technical replicates of each biological replicate were used. RT-qPCR protocols were: initial ...
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Intracellular cytokine staining For intracellular staining of IL-12 p40 (C15.6; BioLegend) cells were stimulated with poly I: C (1 µg/mL; VWR) in the presence of Brefeldin A (BioLegend) for 5 h. Surface markers were stained before fixation and permeabilization with the BD Cytofix/...
Biophysicist with expertise in bioinformatics, population genomics, and biopolymer physics. - apsteinberg
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The membrane was incubated for 1 h in blocking solution (5% nonfat dry milk (Bio-Rad, 1,706,404) in TBS (Bio-Rad, 1,706,435)-0.1% Tween 20 (Bio-Rad, 1,706,531) buffer, followed by incubation with the appropriate primary antibody in blocking solution. After incubation with primary ...