We established the following criteria for the introduction of an automated ANA screening: (1) Positive results must be confirmed by IFA to characterize the pattern and titer of the antibody. (2) Negative results are issued with a notice to request a new test by IFA when the clinical ...
Twitter Google Share on Facebook antinuclear antibody Also found in:Thesaurus,Medical,Acronyms,Encyclopedia. n.Abbr.ANA Any of various antibodies that react with nuclear antigens such as nucleic acids and histones and are often present in the blood of people with systemic autoimmune rheumatic diseases...
ANA was detected by the indirect immunofluorescence assay on HEp2 cells (HEp2-IFA), and ANAs, including 13 antibodies (anti-U1 ribonucleoproteins (anti-U1RNP), anti-Sm antibody, anti-nucleosome antibody (AnuA), anti-ribosome ribonucleoprotein antibody (anti-rRNP), anti-dsDNA antibody, anti-hist...
However, the IIF method is burdened by some unfavorable features: the need for expert morphologists, the subjectivity of interpretation and a low degree of standardization and automation [4], [5], [8]. As a consequence, IIF is considered labor-intensive and prone to render bias. During the...
Performance surveyBackground To evaluate the interpretation and reporting of antinuclear antibodies (ANA) by indirect immunofluorescence assay (IFA) using HEp-2 substrates based on common practice and guidance by the International Consensus on ANA patterns (ICAP). Method Participants included two groups ...
Use of Computer-Aided Immunofluorescence Microscopy (CAIFM) for Interpretation of Antinuclear Antibody (ANA) Pattern and TiterFluorescent Antinuclear AntibodiesImmunofluorescence MicroscopyAutoimmune Diseasecomputer-assistedinterpretationThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA)...