polyspecific reagent and anti-IgGSummary The detection of irregular antibodies is usually performed with serum by the indirect antiglobulin test (IAT) with a polyspecific antiglobulin reagent. In a first study in 1996, we compared the results obtained with 3.264 blood samples of patients drawn ...
AIHA病人产生针对自身红细胞的抗体,这些抗体同激活的补体一起,或只是补体结合到循环血液中自身红细胞膜上,包括抗体和补体共同结合了红细胞,但是抗体又从红细胞解离,补体在这些情况都损伤红细胞膜,红细胞直接溶血或存活期缩短。有研究统计,...
The IgM-IgG complex was isolated by chromatography on DEAE-cellulose and purified by gel-filtration on Sephadex G 200. The IgM in the complex behabed liked a Waldenstrm macroglobulin. The anticardiolipin activity was blocked by saturation with an anti-IgM antiserum, but not with an anti-IgG ...
The preparation of test cells coated with specific IgG of known subclass is described. Such cells are required in the standardization of IgG subtyping reagents. At present, these test cells usually are prepared by coating cells with IgG myeloma paraproteins. However, these paraproteins may not be...
An antiglobulin reagent containing anti-IgG, -C4c, -C4d, -C3c, and -C3d was used to test red blood cells from healthy subjects and from patients in hospital. In tests read macroscopically on opal tiles, no positive reactions were found in the healthy subjects. Positive reactions were, how...
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Il test dell'antiglobulina diretto (Coombs diretto) viene utilizzato per determinare se sulla membrana dei globuli rossi siano presenti anticorpi (IgG) agglutinanti i globuli rossi o il complemento (C3). I globuli rossi del paziente vengono incubati con anticorpi rivolti contro IgG e ...
in which method the serum of a patient with a carcinoma is incubated with CEA that has been radioactively labeled and anti-goat immunoglobulin serum in toto or depleted of the cross-reaction capacity for human IgA, IgG and IgM is added, then the whole mass is incubated and centrifugated, th...
4. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß Anti-Human-IgG, aggregiertes humanes IgG oder ein gamma-Fc-Fragment zugesetzt wird. 5. Verfahren nach Anspruch 1, dadurch gekennzeichnet, daß einer oder beide Antikörper monoklonale Antikörper oder ein Fragment monoklonaler...
A sandwich method was developed for the detection of carcinoembryonic antigen (CEA) and CEA-like molecules and immunoglobulin G (IgG) containing immune complexes (CEA-IgG-IC) in human sera. Rabbit anti-CEA (Dako, FRG) was adsorbed to polypropylene tubes. CEA-like molecules and IgG containing ...