1983. Expansion of antigen-specific T cells from cerebrospinal fluid of patients with multiple sclerosis. J Neuroimmunol 5:317-324.Richen JR, McFarlin DE, Rose JW, et al: Expansion of anrigen- specific T cells from cerebrospinal fluid of patients with multiple sclerosis.J Neuroimmunol 5:3 17...
The adoptive transfer of naive CD4+ T cell antigen receptor (TCR) transgenic (Tg) T cells, labeled with the fluorescent vital-dye CFSE, was used to visualize T cell clonal expansion in vivo. Subcutaneous administration of antigen in CFA containing killed H37Ra induced accumulation and division ...
The present invention encompasses a method for expanding an antigen specific T cell from a population of cells. The method of the present invention comprises contacting a population of cells with an MHC restricted antigenic peptide, a cytokine and a co-stimulatory signal. The invention also ...
Antigen-specific CD4 T cell clonal expansion and differentiation in the aged lymphoid microenvironment. II. The memory T cell response is diminished. Mech Ageing Dev. 2004; 125 :59–68. doi: 10.1016/j.mad.2003.10.003. [ Cross Ref ]
et al. Single cell assessment of allergen-specific T cell responses with MHC class II peptide tetramers: methodological aspects. Int. Arch. Allergy Immunol. 146, 99–112 (2008). Article CAS PubMed Google Scholar Tran, D. Q. et al. Selective expression of latency-associated peptide (LAP)...
T cells after transfer. Large scaleex vivoT-cell expansion and effector differentiation can lead to robust antigen-specific cytolysisin vitro, but also to terminal effector differentiation and poor capacity to further expand and persistin vivo. Accumulating evidence from both animal and human studies...
In culture, interacting T cells assemble the individual APC-ms microrods into a biodegradable 3D matrix. Compared to conventional methods, APC-ms facilitates several-fold greater polyclonal T-cell expansion and improved antigen-specific enrichment of rare T-cell subpopulations. Here we provide a ...
Expansion and functional maturation of human tumor antigen-specific CD8+ T cells after vaccination with antigenic peptide. Peptide-based vaccines are currently being tested for their ability to induce or augment tumor antigen (Ag)-specific CD8+ T-cell responses in cancer patien... MJ Pittet,DE ...
Efficient Induction and Expansion of Human Alloantigen-Specific CD8 Regulatory T Cells from Naive Precursors by CD40-Activated B Cells Although recent studies have focused on CD4 + regulatory T cells (Treg), CD8 + Treg have also been reported to play important roles in the induction and ma......
The efficiency of expansion depends on the density of stimulatory cues and the amount of material in the starting culture. Following a single stimulation, APC-ms enables antigen-specific expansion of rare cytotoxic T-cell subpopulations at a greater magnitude than autologous monocyte-derived dendritic ...