Cryogenic machining is one of the most commonly used techniques for processing and preserving in food industry, and traditional antifreeze agents cannot regulate the mechanical stress damage caused by ice cryst
Co-culturing with cells for 3 h before cryopreservation, 6 % L-Pro +7 % GelMA had the highest survival rate, reaching up to 80 %. Differential Scanning Calorimetry (DSC) analysis showed that 6 % L-Pro + 7 % GelMA lowered the freezing point of the solution to −4.2 °C and ...
Therefore, the increased viscosity could explain, in part, reports on the beneficial effects of antifreeze glycoproteins during cryopreservation by vitrification. Reducing the probability for ice nucleation could be also beneficial for the survival of cold-water fish in their natural habitat. Millimolar ...
Antifreeze proteins from polar fish species are remarkable biomacromolecules which prevent the growth of ice crystals. Ice crystal growth is a major problem in cell/tissue cryopreservation for transplantation, transfusion and basic biomedical research, a
Antifreeze proteins have unique effects on ice in frozen solutions. The most striking property of antifreeze proteins is their ability to prevent growth of ice crystals upon cooling and thus they produce a thermal hysteresis, i.e. a separation of the equilibrium freezing point of a solution and ...
Schematic sketch of the ice-binding process: red water molecules represent the hydration shell of the protein, blue water molecules represent ice; light blue areas represent liquid bulk water. The energetic difference between ice growth in solution (top) and ice-binding to the protein (bottom) is...
H. Recombinant Dendroides canadensis antifreeze proteins as potential ingredients in cryopreservation solutions. Cryobiology 68, 411–418 (2014). 25. Lins, R. D., Pereira, C. S. & Hünenberger, P. H. Trehalose–protein interaction in aqueous solution. Proteins: Structure, Function, and ...
Cryopreservation of finfish and shellfish gametes and embryos 4.4Use of antifreeze proteins Antifreeze proteins(AFPs) are extremely efficient at inhibiting ice recrystallization in frozen solutions.Knight and Duman (1986)have proposed that this may be an important function of the proteins in freeze-toler...
In the past, quality maintenance of frozen foods such as ice creams and protection of cells during cryopreservation using antifreeze proteins derived from plants and fishes have been mainly attempted, although none has yet been put to practical use. Regardless of the high effectiveness being expected...
in 1) to obtain a supernatant solution;3) carrying out heat treatment for the supernatant solution obtained in 2);4) obtaining a supernatant solution containing antifreeze proteins by removing the precipitate generated in 3) by centrifugation; and5) collecting the antifreeze proteins from the ...