It is not obvious whether antibody information from patent documents, such as antibody sequences, is useful in conveying engineering know-how, rather than as a legal reference only. To assess the utility of patent data for therapeutic antibody engineering, we quantified the amount of antibody ...
Thus, obtaining confident and accurate peptide sequences de novo in high throughput is very challenging. Furthermore, even if the peptide was correctly sequenced de novo, inference to its parent protein is, again, strictly dependent on matching the peptide to a known protein in a database, ...
However, to enable effective use and sharing of existing sdAbs, including those with engineered functions ( e.g., fusions with fluorescent proteins), as well as the rational design and engineering of new sdAbs, it is necessary to have access to sequences and experimental data. We have ...
Because these sequences result in a structure that is basically complementary to the shape of the specific antigen bound by the Ig, the hypervariable regions are also called complementarity-determining regions (CDRs). The CDRs project from the relatively flat surfaces of the framework regi...
maintaining the parental germline, the CDR-L2 was identical to the parental, whereas the CDR-L1 and CDR-L3 had 2–3 amino acid differences. For the remaining four antibodies with different light chain germline, all VLCDR sequences were significantly different from the parent antibody sequence. ...
U mutations by a fused deaminase43,44. MAZTER seq relies on the methylation-sensitive ribonuclease and bioinformatic alignment of cleaved versus uncleaved sequences at its target ACA sites45,46. m6A-SEAL uses m6A-specific methyl oxidation by FTO for further derivatization and enrichment47. m6A-REF...
Antibodies currently represent the most valuable category of biopharmaceuticals for both diagnostic and therapeutic applications. They are a class of Y-shaped proteins capable of specifically recognizing and binding to a virtually infinite number of antigens. Being able to identify the antigen-binding res...
abYsis: Integrated Antibody Sequence and Structure—Management, Analysis, and Prediction abYsis is a web-based antibody research system that includes an integrated database of antibody sequence and structure data. The system can be interrogated... MB Swindells,CT Porter,M Couch,... - 《Journal of...
Recombinant phagemids were isolated from each of the 40 colonies and digested by Sfi I and Not I and then ligated with pMD18-T vector for DNA sequencing. Sequence analysis demonstrated that the sequences of the 40 colonies were the same. The number of amino acids and complementary determining...
the training data are generated uniformly at random from the candidate sequence.bThis training data combined with publicly available protein sequences is used to train, refine and evaluate ML models that drive the in silico sequence design process.cThe designed libraries are experimentally validated, ...