Although the 2019 EULAR/ACR classification criteria for systemic lupus erythematosus (SLE) has required at least a positive anti-nuclear antibody (ANA) titer (≥ 1:80), it remains challenging for clinicians to identify patients with SLE. This study ai
Moreover, we show that mAb198.3 binds the surface of different FAT1-positive colon cancer cell lines and it is efficiently internalised, a property that makes it suitable for the development of antibody–drug conjugates (ADC) for CRC therapy. Finally, naked mAb198.3 also shows antitumour ...
g–j ICD induction in CT26 cells treated with IOX1 or its combinations: (g) CLSM images of the CRT exposure (green) and the nuclear HMGB1 (green); blue: DAPI; scale bars, 10 μm; (h) flow cytometric analysis of CRT-positive cells (gated on the propidium iodide negative cells (PI-...
To investigate the precise GAG modifications as well as the overall genetic signature which mediates the binding specificity of bel-sar, a large, multi-tumor type binding screen of bel-sar on 124 cancer cell lines was performed in vitro, where we identified a strong relationship between drug bin...
The reaction was monitored with the presence of controls to eliminate any possible false positive reading (Fig. 9). The limit of detection of the assay was determined using a range of Ubi at 0 mol, 0.05 pmol, 0.002 nmol, 0.01 nmol, 0.02 nmol, 0.05 nmol, 0.2...
Microcrystals of ecTRAIL-R2/KMTR2-Fab were obtained from condition 7 in PEG/Ion Screen 1. Crystallization conditions were finally refined to 75 mM calcium chlo- ride dihydrate (pH 5.1) containing 7.5% (w/v) PEG3350 to yield prism-shaped crystals with a dimension of Scientific Reports | 5:...
Levamisole is an anti-helminthic drug with immunomodulatory properties that is added to cocaine to increase its potency and weight. Levamisole-adulterated cocaine (LAC) may cause an antineutrophil cytoplasmic antibody (ANCA)-associated systemic small ves
6. Enrichment with Positive Clones and Hit Rate The screening for positive clones after the last round of selection was performed using direct ELISA with the targets. Bovine serum albumin (BSA) or milk was used as specificity controls in most of the selections. The number of clones assayed ...
ELISpot B cell ELISpot was performed as previously described with some modifications [20]; 5 × 105 lymphocytes from lungs or splenocytes were added to MultiScreenHTS-HA filter plates (Millipore, Billerica, MA, USA) uncoated or coated with 10 µg/mL A/PR/8 subunit vaccine. Plates were...
2.4. ELISPOT Assay MultiScreen-IP plates (Millipore, Billerica, MA, USA) were coated with the T15 anti-idiotype IgG AB1-2 (15 µg/mL in PBS), or 4 µg/mL MDA-LDL, Cu-OxLDL, or rat anti-mouse IgM polyclonal antibodies (eBioscience) overnight at 4 ◦C, followed by blocking ...