In summary, ARX517 elicited highly specific, potent cell killing in cell lines with high PSMA expression, inhibited tumor growth in enzalutamide-sensitive and enzalutamide-resistant CDX and PDX models, demonstrated a tolerable safety profile in cynomolgus monkeys, and has a clear therapeutic index base...
High sensitivy and specificity. We were using a anti mouse-GAPDH antibody and this anti rabbit-GAPDH antibody gave us more flexibility for probing the blot. Applications:Western Blot Primary Antibody Dilution:1:1000 Cell Tissue Type:Hek293 ...
E3 ubiquitin ligases also exhibited increased expression inApc−/−organoids (Fig.1b). Focusing on E3 ubiquitin ligases with predicted transmembrane domains, only RNF43 and ZNRF3 displayed a similar pattern of increased expression in human colon adenomas compared with normal tissue (Extended Data ...
WesternDot fluorescently labeled secondary antibodies deliver superior signal-to-noise ratios and exhibit comparable sensitivity to enhanced chemiluminescence (ECL)-based methods in western blot applications. No stripping and re-probing or multiple blots are required for multiple protein detection as commonly...
in modulating, directing and amplifying intracellular communications. Beyotime has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways. Many critical protein kinases can be regulated by phosphorylation at a ...
(w/v) in PBS. The plate was incubated at 37 oC for 1 h and washed 3 × 3 with PBST and PBS. The binding was detected using mouse anti-His mAb (Thermo Scientific, Rockford, IL) diluted 1/2000 in 1% (w/v) BSA-PBST and subsequently probing the reaction with 1/2000 ...
Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding ...
it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP...
Figure 1. hSFRP2 mAb reduces metastatic osteosarcoma growth in vivo. (A) Characterization of two osteosarcoma cell lines: RF420 (left) and RF577 (right) by Western blot analysis probing for SFRP2, FZD5, CD38 and PD-L1 levels. Loading control: actin and GAPDH. Western blot shows SFRP2...
Stripping and re-probing of Western blots offers several advantages: 1) Conservation of samples that are expensive or available only in limited quantities, 2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies, 3) Re-analysis of anomalous ...