11. Incubate sections inDAB working solutionuntil desired stain intensity develops. (See Note 2) 12. Rinse sections in tap water. 13. Counterstain, clear and mount. NOTES 1. Solutions containing sodium azide or other inhibitors of peroxidase activity should not be used ...
1、分光光度计/酶标仪/半自动生化分析仪(比色测定波长为550nm) 2、恒温水浴箱或气浴箱 (孵育温度为37℃) 3、台式离心机 4、漩涡混匀器 5、微量移液器 样本收集与前处理: 血清(浆)可直接测定。 红细胞:需按照试剂盒中说明书上红细胞的测定方法进行提取后测定。
After washing, the TUNEL assay was performed according to the manufacturer’s instructions (Abcam, USA; TUNEL Assay Kit-HRP-DAB (ab206386). The sections were then rinsed with PBS, counterstained with hematoxylin, and coverslipped before analysis by light microscopy. Stained slides were scanned by...
the following primary antibodies were used: anti-PSD95, anti-synaptophysin or anti-C1q antibodies, followed by corresponding secondary antibodies conjugated to Alexa Fluor 488 or 594, respectively. Images were captured by Leica TCS SP8 confocal microscope. For 3′-Diaminobenzidine (DAB) immunostaining...
(Product # 13-2500) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counter...
1:200-1:1,000 - 酶联免疫吸附实验 (ELISA) 1:10,000 - 产品规格 种属反应 Human, Mouse 已发表种属 Mouse 宿主/亚型 Mouse / IgG1 分类 Monoclonal 类型 Antibody 克隆号 1A5 抗原 Purified recombinant fragment of human BDH1 expressed in E. Coli. 偶联物 Unconjugated ...
图:1/4 Aurora A Antibody (MA5-15804) in IHC (P) Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA monoclonal antibody (Product # MA5-15804) followed with DAB staining.View more ...
(Product # 39-2500) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were ...
1:500-1:2,000 - 免疫组化(石蜡) (IHC (P)) 1:200-1:1,000 - 酶联免疫吸附实验 (ELISA) 1:10,000 - 产品规格 种属反应 Human 宿主/亚型 Mouse / IgG1 分类 Monoclonal 类型 Antibody 克隆号 5D12C6 抗原 Purified recombinant fragment of human SEC31A (AA: 429-571) expressed in E. coli....
Metal Enhanced DAB Substrate Kit Pierce 34065 275 ML 1460 SuperSignal West Dura Substrate/SuperSignal West Dura持久性化学发光底物 Pierce 34075 100 ML 2980 SuperSignal West Pico Substrate Pierce 34077 100 ML 874 SuperSignal West Pico Substrate Pierce 34078 1 L 4213 SuperSignal West Pico Substrate Pierc...