Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol Storage: GAPDH PAB is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. ...
tabacum leaf extracts have little or no anti-IgG proteolytic activity in the pH range commonly used for experimental extraction. In addition, the use of a broad acting cocktail of protease inhibitors at high concentration had little effect on the IgG fragment pattern for MAb 2G12. These findings...
Each lot of this antibody is quality control tested byWestern blotting. For Western blotting, the suggested use of this reagent is 0.2 - 0.5 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performa...
For those kits, please see instructions on how to adjust protein concentration for optimal dye:protein ratios. **FC=flow cytometry; IF=immunofluorescence; WB=western blot analysis; HCA=high content analysis Not looking for a kit or need a different dye? For flow cytometry antibody conjugation,...
The conjugate elicits a potent and FcyR-dependent activity at low nM concentration. In co-culture of human blood cells and human CEACAM5+ gastric cancer cells, it was shown to induce phagocytosis activity, associated with increased activation/maturation of both monocytes and dendritic cells. Tusa...
Phospho-Hsp27 (Ser82) Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,...
The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting. We recommend using PBS with 1% milk as the antibody diluent and using a high pH CAPS/PVDF transfer protocol. ...
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31437 has been successfully used in Western blot, and ICC applications. Antibody Specificity: This antibody reacts with the heavy chains of mouse IgG, but not with the light chains com...
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Knockout Validated, Western Blot Label DyLight 680 (Excitation = 692 nm, Emission = 712 nm) Antibody Source Monoclonal Mouse IgG2BClone # 159227 Concentration Please see the vial label for concentration. If unlisted please contac...
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration. HostMouse SpecificityRodent Nestin protein confirmed with rat and mouse. Rat-401 is not reactive with human nestin protein. IsotypeIgG1 Species Reactivity ...