Removal or modification of the airliquid interface with a surfactant (e.g., polysorbate) abrogated particle formation. The supernatant postagitation was analyzed using SE-HPLC, FTIR, and AUC analyses and revealed no changes in conformation and aggregation profile when compared to the no...
Removal or modification of the airliquid interface with a surfactant (e.g., polysorbate) abrogated particle formation. The supernatant postagitation was analyzed using SE-HPLC, FTIR, and AUC analyses and revealed no changes in conformation and aggregation profile when compared to the nonagitated ...
ArticleCASGoogle Scholar Demeule, B., Lawrence, M. J., Drake, A. F., Gurny, R. & Arvinte, T. Characterization of protein aggregation: The case of a therapeutic immunoglobulin.Biochim. Biophys. Acta Proteins Proteom.1774(1), 146–153 (2007). 20 May 2021...
Both the RD cell line, the extracellular solution and the intracellular solution were similar to what was used in the QPatch experiments. Again, a nAChR mediated current was elicit by 70 µM ACh, and after compound wash-out, 2 U acetylcholinesterase was added to ensure complete ACh remova...
A soluble antigen is rendered insoluble by aggregation of the Ag/Ab complexes into a lattice. This reaction is the basis ofimmunoelectrophoresis, used e.g. to identify a monoclonal band in myeloma. 2. AGGLUTINATION Particulate antigen, e.g. bacteria and redblood cells, are aggregated in the ...
4. Antibody Aggregation, Stability, and Immunogenicity In high concentrations of formulations for therapy or storage [46], antibodies have been known to aggregate, and the aggregation of therapeutic proteins can lead to immunogenicity [47,48,49]. Numerous experimental studies have been performed to ...
Aggregation of the 42-mer amyloid β-protein (Aβ42) plays a critical role in the pathogenesis of Alzheimer's disease (AD). We have proposed a toxic conformer with a turn at positions 22 and 23, as well as a nontoxic conformer with a turn at positions 25 and 26, in Aβ42 aggregate...
Observations that anti-VEGF antibody-based therapeutics, such as aflibercept and bevacizumab, in complex with VEGF may facilitate platelet aggregation and thrombocyte activation through FcγRIIa in both human and transgenic murine model systems, highlights the importance of considering Fc-mediated effecto...
Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息 CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing ...
F. Physical stability of proteins in aqueous solution: mechanism and driving forces in nonnative protein aggregation. Pharm. Res. 20, 1325–1336 (2003). CAS PubMed Google Scholar Bajaj, H. et al. Protein structural conformation and not second virial coefficient relates to long-term irreversible...