Antibodies Used for Western Blot Analysis.Chera L. MaaroufTyler A. KokjohnDouglas G. WalkerCharisse M. WhitesideWalter M. KalbackAlexis WhetzelLucia I. SueGeidy SerranoSandra A. JacobsonMarwan N. Sabbagh
Potential for non-specific binding that may increase background in certain samples. Host species considerations Multiple species are used to generate antibodies that can be used in western blot applications. Most commonly: mou...
Together, actins are the most abundant proteins in the typical eukaryotic cell, accounting for about 15 percent of total protein in some cell types. As such, actin is widely used as an internal control in Western blotting experiments. Western blot analysis of ACTB in multiple cell line and ...
Find the best loading control for your Western blot using this article, includes: Actin, GAPDH, COX/IV, TBP, tubulin, among others.
However, when labeled anti-IgG, Light Chain Specific antibodies are used for detection; they bind only to the light chain band on the blot (Figure 3) and to light chains on the native primary antibodies used for detection. Therefore, a 50 kDa protein may be detected on blots without interf...
For western blot, the primary antibodies used were a rabbit monoclonal anti phosphorylated eIF2α (Cell Signaling Technologies, Cat. No. 9721) and goat anti-pan-eIF2α (R&D Systems, Cat. No. AF3997) diluted 1:1000 in 5% BSA in TBS with 0.1% Tween-20. The secondary antibodies were a...
应用:Western blot 反应性:人、大鼠、小鼠、猴、犬 别名:AIP1, DRIP4, HP95, PDCD6IP, 程序性细胞死亡6相互作用蛋白抗体。 形态:多克隆抗体,以100微升(2毫克/毫升)的小包装形式供应,包含PBS(磷酸盐缓冲液)、20%甘油和0.05%叠氮钠。该抗体是从山羊抗血清中通过表位亲和层析纯化的。
Western Blot Antibodies: Ready, Set, Reuse Western blotting is the most common method for detecting and quantifying specific proteins. The cost of running a Western is largely driven by detection antibodies, which are used to probe membrane-bound target protein(s). Although some antibodies can be...
western. Of course, we have not tested every antibody in such a manner, since microwestern allows much larger throughput than macro-western. However, these results certainly provide confidence in translation to the traditional western blot. This is particularly satisfying given the much smaller ...
• Most IP antibodies also can be used in Western blot • Better performance for tag IP KIT • IP KIT all the buffers were provided • Validated by multiple cells • Excellent performance for tag IP KIT——Flag/His/GST/GFP/HA/Myc/V5 tag IP KIT ...