Antibodies are secreted immunoglobulin molecules produced mainly by plasma cells. The antigen-binding site of the antibody has a unique structure that allows it to bind antigen in a highly specific manner. Latest Research and Reviews Validation of a multiplexed immunoassay for immunological analysis of...
Furthermore, CHO cell derived sialic acids are mainly in the Neu5Ac form rather than Neu5Gc, and optimization of culture conditions can further decrease the level of Neu5Gc. Using an antisense-RNA strategy, activity reduction of CMP-Neu5Ac hydroxylase, the key enzyme in converting Neu5Ac ...
A comparison of four different ELISA detection systems showed “both sample matrices, blood sera and meat juice, are suitable for antibody detection. However, the test sensitivity mainly depends on the respective cut-off used for the specific test” and “our findings indicate that the currently ...
Polyclonal antibodies are produced by injecting the antigen of interest into an animal and collecting the serum as a source of antibodies some weeks after the immunization. Most commonly used animal species include rabbit, mouse, rat, hamster, and guinea pig. When larger amounts of antibodies are...
These results, together with the immunohistochemistry analysis, demonstrate that the surface of tumor cells is targeted by endogenous IgGs in HGSOC patients. Next, we examined whether the tumor-reactive IgGs are produced by intratumoral ASCs. Immunohistochemistry analysis of tumor samples revealed that...
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This expression is accompanied by the deposition of ligands of ocvβ3, like vitronectin, von Willebrand factor and fibrinogen, and by the anomalous synthesis of such proteins. For example, vitronectin, mainly produced in the liver, is expressed in some tumors. In healthy adults vitronectin and ...
Hantaviruses are etiologic agents of a zoonotic disease transmitted mainly from wild rodents to humans, causing Hemorrhagic Fever with Renal Syndrome in Eurasia and the Hantavirus Cardiopulmonary Syndrome in the Americas (HCPS), reaching a lethality rate of 40% in Brazil. Hantavirus diagnostic and se...
4–6). Contacts are mainly made by CDR-L1 (L1), βC″ strand, βD strand and CDR-L2 (L2) to the VP3 BC loop and HI loop and the VP2 HI loop (Fig. 5). In addition L3 also contacts the VP2 HI loop. Engagement of 38-3-11A Fab with EV71 The variable domains of 38-3-...
The evanescent wave produced by attenuated total reflection (ATR) allows probing of the surface layer of resin beads (50–150 μm diameter), but relies on an intimate contact between the internal reflection element and the sample. Since agarose beads are convex microspheres, only a small ...