Thepth4∆pth3∆mutant (represented by ∆∆) was transformed with the plasmid pRS316 harboring a genomic region including PTH4 and PTH3, termed pPTH4 and pPTH3, and the resultant strains were labeled ∆∆+pPTH4 and ∆∆+pPTH3, respectively. Each strain was diluted and spotted ...
During the infection by contact phase of the infection trial, two affected geckos (a male and a female) were initially individually co-housed for ten days with a disease-free gecko of the opposite sex. The two affected geckos were then co-housed with a single disease-free gecko of the sam...
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PCP domains must be post-translationally modified by the addition of the 4′-phosphopantetheine (PPT) to a conserved serin residue of the conserved GGXS motif [35]. The PCP domain does not play an active role in catalysis, but it has a central role in NRPS function. The PCP interacts ...