The method includes several steps including obtaining a bacterial sample; identifying the type of bacteria in the bacterial sample; selecting a set of antibiotics based on the identity of the bacteria in the bacterial sample; obtaining a control sample from the bacterial sample; placing the ...
Disc diffusion in agar is a common method of testing the sensitivity of bacteria to antibiotics. A microorganism is judged sensitive or resistant according to the diameter of the zone of inhibition of cultural growth, which is then corre... LB Senterfit - Methods in Mycoplasmology 被引量: 46...
an in vitro test for the effect of external conditions on the growth characteristics of bacterial cells. Such tests in particular include tests for the sensivity of a bacteria to an antibiotic or a biostatic agent, and tests to assess the growth stage and health of the bacteria. Methods of...
methods testing for synergy and antagonism with different antibiotics is crucial. The E-test method is rapid, easy to replicate, and can be used to investigate any synergistic potential of combination therapies. The broth dilution method can also be assessed to predict bactericidal...
Are there other ways of testing for resistance? Sometimes molecular methods are used to look for changes (mutations) in a microbe’s genetic material that enables it to grow in the presence of certain antimicrobial drugs. For instance, methicillin-resistant Staphylcoccus aureus (MRSA) contain the...
(Finch et al., 2012). It might also be used to clarify the results of phenotypic β-lactamase confirmation tests and for cases when the results of AR testing are needed immediately (EUCAST, 2013). However, phenotypicASTmethods are referred to as the “gold standard” in diagnostic testing ...
Examining single cells has two key advantages over other testing methods, say the researchers. First, it is rapid, cutting days off the time for typical culture-based assays. It often takes days for a doctor to receive information on resistance, and this means that patients with life-threatenin...
Antibiotic susceptibility testing of Bifidobacterium thermophilum and Bifidobacterium pseudolongum strains: Broth microdilution vs. agar disc diffusion assay There is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bi...
MBT-ASTRA: A suitable tool for fast antibiotic susceptibility testing? The increasing resistance to antibiotics is an urgent health care problem. Detection of resistant microorganisms is the pre-requisite for initiating an ade... Katrin Sparbier a,Sren Schubert b,Markus Kostrzewa a - 《Methods》 ...
Blood culture is the first step in the current standard methods used to diagnose bacteremia. At least 12 h are needed to allow bacterial multiplication providing a rich source of live bacteria for downstream bacteria identification and antibiotic susceptibility testing (AST). Clinical and Laboratory ...