The kanamycin-resistant colonies (named as M. xanthus c7-6E TA-KanR) appeared after seven days and were verified by colony PCR using specific primers to detect the integration of c7-6E TA-KanR into the chromosome of M. xanthus ATCC25232 (Supplementary Fig. S6a). The expected homologous ...
Since the discovery of streptomycin, actinomycetes have been a useful source for new antibiotics, but there have been diminishing rates of new finds since the 1960s. The decreasing probability of identifying new active agents led to reduced interest in s