抑菌圈试验(Antibiotic Disk Diffusion Test)，又称为琼脂扩散法(Agar DiffusionMethod)，是用于评估抗菌药物对特定微生物抑制效果的常用方法。该试验通过测量抗菌药物在琼脂平板上扩散后形成的抑菌圈的直径，来判断药物的抑菌活性。以下是抑菌圈试验的关键步骤和应用：1. 材料准备 培养基：常用的培养基是Muller-Hinton琼...
Antibiotic susceptibility testing (agar disk diffusion and agar dilution) of clinical isolates of Corynebacterium jeikeium. Chemotherapy 1998; 44: 230±7.Traub WH, Geipel U, Leonhard B, Bauer D. Antibiotic sus- ceptibility testing (agar disk diffusion and agar dilution) of clinical isolates of ...
The researchers used AI to interpret a common laboratory test known as the Kirby-Bauer disk diffusion test, which helps doctors to determine which antibiotics can or can’t fight a particular bacterial infection. Based on GPT-4, the scientists created the “EUCAST-GPT-expert”, which follows st...
C. Standardisation of disk diffusion results for antibiotic susceptibility testing using the sirscan automated zone reader. BMC Microbiol. 13, 225 (2013). Article PubMed PubMed Central CAS Google Scholar Smith, S., Fowora, M. & Pellicano, R. Infections with Helicobacter pylori and challenges ...
In the disk diffusion test and the E test, antibiotics diffuse from special filter disks or strips through an agar medium and create a zone of inhibition if the drug is effective against the bacteria. The E test allows more precise measurement of the minimal inhibitory concentration (MIC)...
Standardisation of disk diffusion readings could improve reproducibility and accuracy of antibiotic susceptibility testing (AST). This study evaluated accuracy, reproducibility, and precision of automated inhibition zone reading using the “Sirscan autom
The disk diffusion method establishes a gradient of antibiotic around an antibiotic disk placed on solid medium, creating a radius around the disk where sensitive bacteria cannot grow [5]. Resistant bacteria will be able to grow closer to the disk, that is, at higher antibiotic concentration, ...
Using the disk diffusion test, MRSA strains show zone diameter ≥17 mm, while VISA strains show ≥ 15 mm61. Though accurate, these techniques lack rapid diagnoses and require ~2–3 days for determining the presence of AMR bacterial species. These techniques have limited potential as a ...
At the same time, the zone edge test applied to disk diffusion plates [55] matched the β-lactamase prediction from both the nitrocefin tests and β-LEAF assay for these isolates (Table 2- columns 2, 3 and 4). Similarly, while the E-tests suggested isolates #1 and #6 to be cefoxitin...
The Balaban Group has developed the Tolerance-Detection Test (TD test) for the easy detection of tolerant and persistent bacteria [64]. The TD test is a derivative of the Kirby-Bauer disk diffusion assay used to assess antibiotic susceptibility. After evaluating a strain's antibiotic ...