Hrp pili subunits (HrpE), an essential component of Type III secretion system (T3SS) bacteria, play a crucial role in the pathogenesis of Xcc by transporting effector proteins into the host cell and causing canker symptoms. Therefore, development of antibodies that block ...
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HRP-conjugated goat anti-human IgG antibody was used to detect hLIF-hFc bound to LIFR or gp130. The results showed that 1G11 could significantly block LIF binding to LIFR, but did not affect LIF binding to gp130. None of the remaining 6 antibodies were observed to block LIF binding to ...
For that, antibodies were labeled with biotin and detected with horseradish peroxidase (HRP)-streptavidin. These immunoblot images are also provided in Supplementary Fig. S4b. MW, molecular weight (kDa). Full size image The aggregation state of the 3F12E7 scFv was assessed by native gel ...
Competitive ELISA was performed to determine if these 7 antibodies could interfere with LIF binding to its receptors LIFR and gp130. Antibodies were incubated with hLIF-hFc and added to the microtiter plate wells coated with LIFR or gp130. HRP-conjugated goat anti-human IgG antibody was used ...
Recombinant C28-GUSh fusion protein was detected using anti-myc antibody 9E10 (Chan et al, 1987), and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG (Dako, Glostrup, Denmark). Blots were developed with Lumilight Plus (Roche).gahuls...
To compare the sensitivity of the Qdot probe with a conventional Western blot, GRP78 protein was diluted in electrophoresis buffer and subjected to Western blot analysis with either Qdot-GRP78 antibody or the goat polyclonal anti-GRP78 antibody with conventional secondary antibody: HRP-conjugated ...