Diagnosis is made with a screening antiplatelet factor (PF4) immunoassay and the serotonin-release assay (SRA) as a confirmatory test. Anti-PF4 assays have high sensitivity (98%) but lower specificity (50%) and result in frequent false-positive tests. We present a rare cas...
Performance characteristics of a rapid assay for anti-PF4/heparin antibodies: the particle immunofiltration assay. J Thromb Haemost 2007;5 (11):2308-2310Warkentin TE, Sheppard JI, Raschke R, Greinacher A. Perform- ance characteristics of a rapid assay for anti-PF4/heparin anti- bodies: the ...
Antiphospholipid and anti-PF4 antibodies: an association affecting anti-PF4/heparin assay analysis 来自 EBSCO 喜欢 0 阅读量: 17 作者: TE Warkentin 摘要: View Full Article (HTML) Get PDF (141K) More content like this Find more content: like this article Find more content written by:T. E. ...
All patients initially had a positive assay for PF4-dependent platelet-activating antibodies and were repeatedly tested (median follow-up of 25 weeks; range, 3-36 weeks). Patients at risk are shown under the graph. Antibody levels in the anti-PF4/heparin IgG EIA decreased over the time of ...
The analysis performed using the 4T's scoring system revealed that 11 of 20 (15.7%) patients with antibodies in at least 1 assay had intermediate or greater probability of HIT. Patients without antibodies had significantly higher levels of D-dimer than those with antibodies. However, there were...
In the first patient, clinical judgment rejected the suspicion of HIT despite a positive screening assay, and treatment for the alternative diagnosis of post-transfusion purpura was correctly initiated. In the second patient, the inaccurate diagnosis HIT was pursued due to a positive screening assay,...
Anti-PF4-heparin immunoglobulin G is the major class of heparin-induced thrombocytopenia antibody: findings of an enzyme-linked immunofiltration assay using membrane-bound hPF4-heparin. Br J Haematol. 2001;112:69-75.Vun CM, Evans S, Chesterman CN. Anti-PF4-heparin immuno- globulin G is the ...
To evaluate performance of the assay, we conducted precision, accuracy, and qualitative method comparison. We compared the assay of interest with two reference lab immunoassays and the gold standard reference method (serotonin release assay). The statistical analysis was carried out in EP Evaluator ...
Anti-HPF4 antibody assay was performed for all eligible patients by ELISA method. Statistical significance was based on two-sided design-based tests evaluated at the 0.05 level of significance. Most of the patients (n=57, 58.8%) were male. The mean age of the patients was 55.46±15.2years,...
Antiphospholipid and anti-PF4 antibodies: an association affecting anti-PF4/heparin assay analysis 来自 NCBI 喜欢 0 阅读量: 20 作者: TE Warkentin 摘要: Michael G. DeGroote School of Medicine, McMaster University, Hamilton, ON, Canada. twarken@mcmaster.ca 关键词: calcium platelet store-operated ...