Briefly, a sample spiked with monoclonal human IgG anti-PEG antibody is diluted in buffer and incubated with paramagnetic beads coated with linear chain mPEG to capture anti-PEG antibodies. The complex is then captured on an acoustic membrane coated with Protein A. The change in mass on the ...
molecules Article Understanding the Role of Anti-PEG Antibodies in the Complement Activation by Doxil in Vitro Barry W. Neun 1, Yechezkel Barenholz 2, Janos Szebeni 3,4,5 and Marina A. Dobrovolskaia 1,* ID 1 Nanotechnology Characterization Lab, Frederick National Laboratory for Cancer ...
Anti-PEG IgE In Anaphylaxis Associated with Polyethylene Glycol demonstrated by an in vitro assay-- dual cytometric beads assay. IgE Type 1 hypersensitivity is a mechanism of PEG associated anaphylaxis. This new detection method could be a useful tool both for clinical practice and in screening ...