binding assaysenzymoimmunoassaysimmunochemical methodsradioimmune assaysdoi:10.1096/fasebj.4.9.2347467Varga, J MKlein, G FFritsch, PThe Federation of American Societies for Experimental BiologyThe FASEB JournalVarga JM, Klein GF, Fritsch P (1990) Binding of a mouse monoclonal IgE (anti-DNP) ...
The number of combining sites per mole of bovine colostral anti-DNP IG2 was found to be 2 and the total affinity constant was 0.81 × 104M-1 Unlike bovine colostral IgG1, the nonspecific binding of H3-ε-DNP-1-lysine by IgG2 as a function of increased concentrations, did not show ...
Kinetics of Multivalent Antigen DNP-BSA Binding to IgE-FcεRI in Relationship to the Stimulated Tyrosine Phosphorylation of FcεRI Multivalent DNP-BSA is commonly used to cross-link anti-DNP IgE bound to Fc epsilon RI to stimulate cellular responses, although key features of the bindin... K...